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Optimising Elicitors and Precursors to Enhance Alizarin and Purpurin Production in Adventitious Roots of Morinda citrifolia L.

Optimising Elicitors and Precursors to Enhance Alizarin and Purpurin Production in Adventitious... Morinda citrifolia L. belongs to family Rubiaceae. It is a traditional Polynesian medicinal plant. Its root serves as a rich source of various anthraquinone constituents. The anthraquinone compounds exhibit therapeutic effects of antibacterial, antiviral and anticancer activities. In the present work, an adventitious root culture of M. citrifolia was established and the effectiveness of various elicitors viz, yeast extract, xylan, pectin and the precursor α-ketoglutaric acid on the level of anthraquinone production in adventitious root cultures were assayed. Adventitious root proliferation was achieved in Murashige and Skoog liquid medium supplemented with 24 μM Indole-3-butyric acid. The anthraquinone content was determined by spectrophotometric analysis and the anthraquinone constituents–viz, alizarin and purpurin were quantified by high-performance liquid chromatographic method. Enhancement in alizarin and purpurin along with total anthraquinones was detected due to the addition of precursor and elicitors. Pectin (20 mg/l) added cultures resulted in highest anthraquinone accumulation (44.4 mg/g dw). The protocol developed for the regeneration of roots was simple, reproducible and reliable for the possible commercial production of in vitro root biomass based alizarin and purpurin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Optimising Elicitors and Precursors to Enhance Alizarin and Purpurin Production in Adventitious Roots of Morinda citrifolia L.

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References (28)

Publisher
Springer Journals
Copyright
Copyright © 2014 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-014-0395-7
Publisher site
See Article on Publisher Site

Abstract

Morinda citrifolia L. belongs to family Rubiaceae. It is a traditional Polynesian medicinal plant. Its root serves as a rich source of various anthraquinone constituents. The anthraquinone compounds exhibit therapeutic effects of antibacterial, antiviral and anticancer activities. In the present work, an adventitious root culture of M. citrifolia was established and the effectiveness of various elicitors viz, yeast extract, xylan, pectin and the precursor α-ketoglutaric acid on the level of anthraquinone production in adventitious root cultures were assayed. Adventitious root proliferation was achieved in Murashige and Skoog liquid medium supplemented with 24 μM Indole-3-butyric acid. The anthraquinone content was determined by spectrophotometric analysis and the anthraquinone constituents–viz, alizarin and purpurin were quantified by high-performance liquid chromatographic method. Enhancement in alizarin and purpurin along with total anthraquinones was detected due to the addition of precursor and elicitors. Pectin (20 mg/l) added cultures resulted in highest anthraquinone accumulation (44.4 mg/g dw). The protocol developed for the regeneration of roots was simple, reproducible and reliable for the possible commercial production of in vitro root biomass based alizarin and purpurin.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Aug 27, 2014

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