Get 20M+ Full-Text Papers For Less Than $1.50/day. Subscribe now for You or Your Team.

Learn More →

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated... Acta Pharmacologica Sinica (2015) 36: 298–310 npg © 2015 CPS and SIMM All rights reserved 1671-4083/15 $32.00 www.nature.com/aps Original Article Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A receptor-mediated transactivation of epidermal growth factor receptor Min ZHONG, Wan-ling SONG, Ye-chun XU, Yang YE, Lin-yin FENG CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China Aim: Paeoniflorin from Chinese herb Paeoniae Radix has been shown to ameliorate middle cerebral artery occlusion-induced ischemia in rats. The aim of this study was to investigate the mechanisms underlying the neuroprotective action of PF in cultured rat cortical neurons. Methods: Primary cultured cortical neurons of rats were subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) insult. Cell survival was determined using MTT assay. HEK293 cells stably transfected with A R (HEK293/A R) were used for detailed 1 1 analysis. Phosphorylation of the signaling proteins was evaluated by Western blot or immunoprecipitation. Receptor interactions were identified using co-immunoprecipitation and immunofluorescence staining. Results: Paeoniflorin (10 nmol/L to 1 μmol/L) increased the survival of neurons subjected to OGD/R. Furthermore, paeoniflorin increased the phosphorylation of Akt and ERK1/2 in these neurons. These effects were blocked by PI3K inhibitor http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Pharmacologica Sinica Springer Journals

Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A1 receptor-mediated transactivation of epidermal growth factor receptor

Loading next page...
 
/lp/springer-journals/paeoniflorin-ameliorates-ischemic-neuronal-damage-in-vitro-via-G6sqs5MfA4

References (42)

Publisher
Springer Journals
Copyright
Copyright © 2015 by CPS and SIMM
Subject
Biomedicine; Biomedicine, general; Pharmacology/Toxicology; Medical Microbiology; Immunology; Internal Medicine; Vaccine
ISSN
1671-4083
eISSN
1745-7254
DOI
10.1038/aps.2014.154
Publisher site
See Article on Publisher Site

Abstract

Acta Pharmacologica Sinica (2015) 36: 298–310 npg © 2015 CPS and SIMM All rights reserved 1671-4083/15 $32.00 www.nature.com/aps Original Article Paeoniflorin ameliorates ischemic neuronal damage in vitro via adenosine A receptor-mediated transactivation of epidermal growth factor receptor Min ZHONG, Wan-ling SONG, Ye-chun XU, Yang YE, Lin-yin FENG CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China Aim: Paeoniflorin from Chinese herb Paeoniae Radix has been shown to ameliorate middle cerebral artery occlusion-induced ischemia in rats. The aim of this study was to investigate the mechanisms underlying the neuroprotective action of PF in cultured rat cortical neurons. Methods: Primary cultured cortical neurons of rats were subjected to oxygen-glucose deprivation and reoxygenation (OGD/R) insult. Cell survival was determined using MTT assay. HEK293 cells stably transfected with A R (HEK293/A R) were used for detailed 1 1 analysis. Phosphorylation of the signaling proteins was evaluated by Western blot or immunoprecipitation. Receptor interactions were identified using co-immunoprecipitation and immunofluorescence staining. Results: Paeoniflorin (10 nmol/L to 1 μmol/L) increased the survival of neurons subjected to OGD/R. Furthermore, paeoniflorin increased the phosphorylation of Akt and ERK1/2 in these neurons. These effects were blocked by PI3K inhibitor

Journal

Acta Pharmacologica SinicaSpringer Journals

Published: Feb 9, 2015

There are no references for this article.