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Programmable and printable Bacillus subtilis biofilms as engineered living materials

Programmable and printable Bacillus subtilis biofilms as engineered living materials Bacterial biofilms can be programmed to produce living materials with self-healing and evolvable functionalities. However, the wider use of artificial biofilms has been hindered by limitations on processability and functional protein secretion capacity. We describe a highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis. We demonstrate that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties. Our engineered biofilms have the viscoelastic behaviors of hydrogels and can be precisely fabricated into microstructures having a diversity of three-dimensional (3D) shapes using 3D printing and microencapsulation techniques. Notably, these long-lasting and environmentally responsive fabricated living materials remain alive, self-regenerative, and functional. This new tunable platform offers previously unattainable properties for a variety of living functional materials having potential applications in biomaterials, biotechnology, and biomedicine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Chemical Biology Springer Journals

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References (51)

Publisher
Springer Journals
Copyright
Copyright © 2018 by The Author(s), under exclusive licence to Springer Nature America, Inc.
Subject
Chemistry; Chemistry/Food Science, general; Biochemical Engineering; Biochemistry, general; Cell Biology; Bioorganic Chemistry
ISSN
1552-4450
eISSN
1552-4469
DOI
10.1038/s41589-018-0169-2
Publisher site
See Article on Publisher Site

Abstract

Bacterial biofilms can be programmed to produce living materials with self-healing and evolvable functionalities. However, the wider use of artificial biofilms has been hindered by limitations on processability and functional protein secretion capacity. We describe a highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis. We demonstrate that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties. Our engineered biofilms have the viscoelastic behaviors of hydrogels and can be precisely fabricated into microstructures having a diversity of three-dimensional (3D) shapes using 3D printing and microencapsulation techniques. Notably, these long-lasting and environmentally responsive fabricated living materials remain alive, self-regenerative, and functional. This new tunable platform offers previously unattainable properties for a variety of living functional materials having potential applications in biomaterials, biotechnology, and biomedicine.

Journal

Nature Chemical BiologySpringer Journals

Published: Dec 3, 2018

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