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RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants

RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants The study was conducted by taking six soil samples, two each for Capparis decidua (Ker), Calligonum polygonoides (Phog) collected from Bikaner while third sample, Cuminum cyminum (Cumin) was collected from Jobner. RAPD analysis was done using various decamer primers. The total bands amplified varied from 7 (UBC 153) to 13 (UBC 157). Three primers (UBC 153, 154 and 162) showed 100 % polymorphism. 46 out of 53 polymorphic allelic positions were obtained with 86.7 % polymorphism. The number of bands generated was plant and soil dependent. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

RAPD Analysis to Study Metagenome Diversity in Soil Microbial Community of Arid Zone Plants

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References (12)

Publisher
Springer Journals
Copyright
Copyright © 2013 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-012-0041-1
Publisher site
See Article on Publisher Site

Abstract

The study was conducted by taking six soil samples, two each for Capparis decidua (Ker), Calligonum polygonoides (Phog) collected from Bikaner while third sample, Cuminum cyminum (Cumin) was collected from Jobner. RAPD analysis was done using various decamer primers. The total bands amplified varied from 7 (UBC 153) to 13 (UBC 157). Three primers (UBC 153, 154 and 162) showed 100 % polymorphism. 46 out of 53 polymorphic allelic positions were obtained with 86.7 % polymorphism. The number of bands generated was plant and soil dependent.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: Apr 10, 2013

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