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Rapid and Efficient Method of Total RNA Isolation from Milk Fat for Transcriptome Analysis of Mammary Gland

Rapid and Efficient Method of Total RNA Isolation from Milk Fat for Transcriptome Analysis of... Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. https://doi.org/10.1007/s40011-017-0955-8 RESEARCH ARTICLE Rapid and Efficient Method of Total RNA Isolation from Milk Fat for Transcriptome Analysis of Mammary Gland 1 1 Shanti Choudhary Ratan K. Choudhary Received: 23 August 2016 / Revised: 27 November 2017 / Accepted: 19 December 2017 The National Academy of Sciences, India 2018 Abstract Isolation of good quality ribonucleic acid (RNA) Introduction from mammary glands of elite animals is often hindered by invasive method of mammary tissue sampling, ethical Invasive procedure of tissue biopsy is sometime challeng- permission, time consuming, and repeated biopsies from ing during pregnancy or early lactation period when gland the same animal at different time points. The aim of the is highly vascularized. Biopsy at this stage creates techni- present study was to optimize a protocol for RNA isolation cal challenge due to excessive blood loss followed by from milk fat that is suitable for transcriptome analysis. In management issues in animal recovery. Investigators have this study, authors isolated good quality RNA from milk fat attempted to isolate total RNA from milk somatic cells of goats and buffalo milk by combining two methods (SC) to analyze gene expression profile of mammary http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Rapid and Efficient Method of Total RNA Isolation from Milk Fat for Transcriptome Analysis of Mammary Gland

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References (12)

Publisher
Springer Journals
Copyright
Copyright © 2018 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-017-0955-8
Publisher site
See Article on Publisher Site

Abstract

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. https://doi.org/10.1007/s40011-017-0955-8 RESEARCH ARTICLE Rapid and Efficient Method of Total RNA Isolation from Milk Fat for Transcriptome Analysis of Mammary Gland 1 1 Shanti Choudhary Ratan K. Choudhary Received: 23 August 2016 / Revised: 27 November 2017 / Accepted: 19 December 2017 The National Academy of Sciences, India 2018 Abstract Isolation of good quality ribonucleic acid (RNA) Introduction from mammary glands of elite animals is often hindered by invasive method of mammary tissue sampling, ethical Invasive procedure of tissue biopsy is sometime challeng- permission, time consuming, and repeated biopsies from ing during pregnancy or early lactation period when gland the same animal at different time points. The aim of the is highly vascularized. Biopsy at this stage creates techni- present study was to optimize a protocol for RNA isolation cal challenge due to excessive blood loss followed by from milk fat that is suitable for transcriptome analysis. In management issues in animal recovery. Investigators have this study, authors isolated good quality RNA from milk fat attempted to isolate total RNA from milk somatic cells of goats and buffalo milk by combining two methods (SC) to analyze gene expression profile of mammary

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Jan 5, 2018

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