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SMP production by activated sludge in the presence of a metabolic uncoupler, 3,3′,4′,5-tetrachlorosalicylanilide (TCS)

SMP production by activated sludge in the presence of a metabolic uncoupler,... 3,3′,4′,5-Tetrachlorosalicylanilide (TCS) is an effective metabolic uncoupler utilized for microbial yield reduction. However, its potential impact, in particular on the soluble microbial products (SMP) formation, is unknown yet. Herein we study the effect of TCS on SMP production and analyze the related mechanism. The addition of TCS in activated sludge system led to an increased production of SMP, especially proteins. The SMP were produced in proportion to the substrate utilization at a low TCS concentration, while more non-substrate-associated SMP were released at a high TCS concentration. TCS simulated the production of extracellular polymeric substances (EPS) and enhanced cell lysis, which both contributed to SMP production. FTIR and EEM analyses show that the SMP, EPS, and cell lysis products have similar functional groups and fluorescence properties, indicating a similar origin of these substances. In addition, a dose of TCS increased the release of high molecular weight compounds due to cell lysis. This study might benefit for a better understanding of the response of activated sludge to metabolic uncouplers like TCS. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Microbiology and Biotechnology Springer Journals

SMP production by activated sludge in the presence of a metabolic uncoupler, 3,3′,4′,5-tetrachlorosalicylanilide (TCS)

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References (52)

Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Chemistry; Microbial Genetics and Genomics; Microbiology; Biotechnology
ISSN
0175-7598
eISSN
1432-0614
DOI
10.1007/s00253-011-3765-6
pmid
22159739
Publisher site
See Article on Publisher Site

Abstract

3,3′,4′,5-Tetrachlorosalicylanilide (TCS) is an effective metabolic uncoupler utilized for microbial yield reduction. However, its potential impact, in particular on the soluble microbial products (SMP) formation, is unknown yet. Herein we study the effect of TCS on SMP production and analyze the related mechanism. The addition of TCS in activated sludge system led to an increased production of SMP, especially proteins. The SMP were produced in proportion to the substrate utilization at a low TCS concentration, while more non-substrate-associated SMP were released at a high TCS concentration. TCS simulated the production of extracellular polymeric substances (EPS) and enhanced cell lysis, which both contributed to SMP production. FTIR and EEM analyses show that the SMP, EPS, and cell lysis products have similar functional groups and fluorescence properties, indicating a similar origin of these substances. In addition, a dose of TCS increased the release of high molecular weight compounds due to cell lysis. This study might benefit for a better understanding of the response of activated sludge to metabolic uncouplers like TCS.

Journal

Applied Microbiology and BiotechnologySpringer Journals

Published: Dec 13, 2011

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