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Statistical Evaluation of Paternity Using Most Recent STR-Based Multiplex Systems

Statistical Evaluation of Paternity Using Most Recent STR-Based Multiplex Systems Short Tandem Repeats (STRs) are widely accepted genetic tool for human identification. The present DNA technique based on STR, Polymerase Chain reaction (PCR) and capillary electrophoresis is the backbone of criminal investigation today. We present here statistical evaluation of a case of parentage determination in a trio match (mother, child and father) using different STR-based assays. DNA profiles were obtained using two 5-dye-based and four most recent 6-dye-based multiplex systems being used for human DNA Identification. All sets of genetic markers tested using different multiplex systems confirmed that the child inherited half of the alleles from the mother and the remaining half from the father. To investigate co-relation with the number of loci in paternity calculation, this study was undertaken using 15, 20, 21, 23 and 24 autosomal STR along with Amelogenin markers in a case of paternity. Parameters of forensic significance are presented for AmpFISTR® Identifiler® Plus, PowerPlex® 21 multiplex system, Global Filer®, Sure ID PanGlobal® Human DNA Identification Kit, PowerPlex® Fusion 6Csystem and VeriFiler™ Plus PCR Amplification kits. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Statistical Evaluation of Paternity Using Most Recent STR-Based Multiplex Systems

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References (16)

Publisher
Springer Journals
Copyright
Copyright © The National Academy of Sciences, India 2021
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-021-01233-2
Publisher site
See Article on Publisher Site

Abstract

Short Tandem Repeats (STRs) are widely accepted genetic tool for human identification. The present DNA technique based on STR, Polymerase Chain reaction (PCR) and capillary electrophoresis is the backbone of criminal investigation today. We present here statistical evaluation of a case of parentage determination in a trio match (mother, child and father) using different STR-based assays. DNA profiles were obtained using two 5-dye-based and four most recent 6-dye-based multiplex systems being used for human DNA Identification. All sets of genetic markers tested using different multiplex systems confirmed that the child inherited half of the alleles from the mother and the remaining half from the father. To investigate co-relation with the number of loci in paternity calculation, this study was undertaken using 15, 20, 21, 23 and 24 autosomal STR along with Amelogenin markers in a case of paternity. Parameters of forensic significance are presented for AmpFISTR® Identifiler® Plus, PowerPlex® 21 multiplex system, Global Filer®, Sure ID PanGlobal® Human DNA Identification Kit, PowerPlex® Fusion 6Csystem and VeriFiler™ Plus PCR Amplification kits.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Sep 1, 2021

Keywords: STR; Paternity index; DNA profile; CRMNE

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