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Stem Cells and Tissue EngineeringPrinciples and Practice of Stem Cell Cryopreservation

Stem Cells and Tissue Engineering: Principles and Practice of Stem Cell Cryopreservation [Recent extensive application of various cell-mediated therapeutic approaches has resulted in increased needs for both specific blood-derived cells and operating procedures to get minimized cell damages during their collection, processing, and storage in liquid or frozen state. The aim of cryoinvestigations is to minimize cell injuries during the freeze/thaw process (cryoinjury). Cryoinjuries may be the result of extensive cell dehydration and/or intracellular ice crystallization. The basic goal of cryopreservation is to maintain the cell viability—which may be defined as the ability of cells to perform their normal or near-normal function when transfused or transplanted. Generally, postthaw cell recovery is superior when the most appropriate freezing procedure and the best cryoprotective agent (cryoprotectant) are used. For blood progenitor or cell cryopreservation, glycerol, dimethyl sulfoxide (DMSO), and hydroxyetilstarch (HES) are regularly used, although in different concentrations. Despite the fact that cell freezing practice is already in routine use, some questions related to the optimal living cell cryopreservation are still unresolved.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Stem Cells and Tissue EngineeringPrinciples and Practice of Stem Cell Cryopreservation

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References (39)

Publisher
Springer New York
Copyright
© The Author(s) 2013
ISBN
978-1-4614-5504-2
Pages
71 –81
DOI
10.1007/978-1-4614-5505-9_13
Publisher site
See Chapter on Publisher Site

Abstract

[Recent extensive application of various cell-mediated therapeutic approaches has resulted in increased needs for both specific blood-derived cells and operating procedures to get minimized cell damages during their collection, processing, and storage in liquid or frozen state. The aim of cryoinvestigations is to minimize cell injuries during the freeze/thaw process (cryoinjury). Cryoinjuries may be the result of extensive cell dehydration and/or intracellular ice crystallization. The basic goal of cryopreservation is to maintain the cell viability—which may be defined as the ability of cells to perform their normal or near-normal function when transfused or transplanted. Generally, postthaw cell recovery is superior when the most appropriate freezing procedure and the best cryoprotective agent (cryoprotectant) are used. For blood progenitor or cell cryopreservation, glycerol, dimethyl sulfoxide (DMSO), and hydroxyetilstarch (HES) are regularly used, although in different concentrations. Despite the fact that cell freezing practice is already in routine use, some questions related to the optimal living cell cryopreservation are still unresolved.]

Published: Oct 1, 2012

Keywords: Cell Recovery; Cell Preservation; Cell Cryopreservation; Cryoprotectant Agent; Cell Dehydration

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