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Structure and transcription of human papillomavirus sequences in cervical carcinoma cells

Structure and transcription of human papillomavirus sequences in cervical carcinoma cells DNA of human papillomavirus (HPV) types 16 and 18 has been found closely associated with human genital cancer1,2, supporting the concept that members of this virus group are key factors in the aetiology of genital cancer3. HPV 18 DNA sequences were also detected in cell lines derived from cervical cancer2. We have now analysed these cell lines, HeLa, C4-1 and 756, for the structural organization and transcription of the HPV 18 genome and we find that the HPV 18 DNA is integrated into the cellular genome and is amplified in HeLa and 756 cells. Almost the complete HPV 18 genome seems to be present in 756 cells, with the early region being disrupted into two portions in each integrated copy. In HeLa and C4-1 cells, a 2–3 kilobase (kb) segment of HPV 18-specific sequences is missing from the E2 to L2 region. HPV 18 sequences are specifically transcribed from the E6–E7–E1 region into poly(A)+ RNAs of 1.5–6.5 kb. Hybridization analysis of cDNA clones indicated that some of the transcripts are composed of HPV 18 and cellular sequences. In addition, poly(A)+ RNA hybridizing with HPV 16 DNA was found in two out of three cervical carcinoma biopsies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Structure and transcription of human papillomavirus sequences in cervical carcinoma cells

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References (19)

Publisher
Springer Journals
Copyright
Copyright © 1985 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/314111a0
Publisher site
See Article on Publisher Site

Abstract

DNA of human papillomavirus (HPV) types 16 and 18 has been found closely associated with human genital cancer1,2, supporting the concept that members of this virus group are key factors in the aetiology of genital cancer3. HPV 18 DNA sequences were also detected in cell lines derived from cervical cancer2. We have now analysed these cell lines, HeLa, C4-1 and 756, for the structural organization and transcription of the HPV 18 genome and we find that the HPV 18 DNA is integrated into the cellular genome and is amplified in HeLa and 756 cells. Almost the complete HPV 18 genome seems to be present in 756 cells, with the early region being disrupted into two portions in each integrated copy. In HeLa and C4-1 cells, a 2–3 kilobase (kb) segment of HPV 18-specific sequences is missing from the E2 to L2 region. HPV 18 sequences are specifically transcribed from the E6–E7–E1 region into poly(A)+ RNAs of 1.5–6.5 kb. Hybridization analysis of cDNA clones indicated that some of the transcripts are composed of HPV 18 and cellular sequences. In addition, poly(A)+ RNA hybridizing with HPV 16 DNA was found in two out of three cervical carcinoma biopsies.

Journal

NatureSpringer Journals

Published: Mar 7, 1985

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