Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Telomerase activation by the E6 gene product of human papillomavirus type 16

Telomerase activation by the E6 gene product of human papillomavirus type 16 ACTIVATION of telomerase, a ribonucleoprotein complex that synthesizes telomere repeat sequences1–4, is linked to cell immortalization and is characteristic of most cell lines and tumours4,5–8. Here we show that expression of the human papillomavirus type 16 (HPV-16) E6 protein activates telomerase in early-passage human keratinocytes and mammary epithelial cells. This activation was observed in cells pre-crisis, that is, before they became immortal, and occurred within one passage of retroviral infection with vectors expressing HPV-16 E6. Studies using HPV-16 E6 mutants showed that there was no correlation between the ability of the mutants to activate telomerase and their ability to target p53 for degradation, suggesting that telomerase activation by HPV-16 E6 is p53 independent. Keratinocytes expressing wild-type HPV-16 E6 have an extended lifespan, but do not become immortal9, indicating that telomerase activation and E6-mediated degradation of p53 are insufficient for their immortalization. These results show that telomerase activation is an intrinsic, but insufficient, component of transformation by HPV. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Nature Springer Journals

Telomerase activation by the E6 gene product of human papillomavirus type 16

Loading next page...
 
/lp/springer-journals/telomerase-activation-by-the-e6-gene-product-of-human-papillomavirus-fwbZ0u811D

References (20)

Publisher
Springer Journals
Copyright
Copyright © 1996 by Nature Publishing Group
Subject
Science, Humanities and Social Sciences, multidisciplinary; Science, Humanities and Social Sciences, multidisciplinary; Science, multidisciplinary
ISSN
0028-0836
eISSN
1476-4687
DOI
10.1038/380079a0
Publisher site
See Article on Publisher Site

Abstract

ACTIVATION of telomerase, a ribonucleoprotein complex that synthesizes telomere repeat sequences1–4, is linked to cell immortalization and is characteristic of most cell lines and tumours4,5–8. Here we show that expression of the human papillomavirus type 16 (HPV-16) E6 protein activates telomerase in early-passage human keratinocytes and mammary epithelial cells. This activation was observed in cells pre-crisis, that is, before they became immortal, and occurred within one passage of retroviral infection with vectors expressing HPV-16 E6. Studies using HPV-16 E6 mutants showed that there was no correlation between the ability of the mutants to activate telomerase and their ability to target p53 for degradation, suggesting that telomerase activation by HPV-16 E6 is p53 independent. Keratinocytes expressing wild-type HPV-16 E6 have an extended lifespan, but do not become immortal9, indicating that telomerase activation and E6-mediated degradation of p53 are insufficient for their immortalization. These results show that telomerase activation is an intrinsic, but insufficient, component of transformation by HPV.

Journal

NatureSpringer Journals

Published: Mar 7, 1996

There are no references for this article.