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Transfer of clover cyst nematode resistance from Trifolium nigrescens Viv. to T. repens L. by interspecific hybridisation

Transfer of clover cyst nematode resistance from Trifolium nigrescens Viv. to T. repens L. by... Resistance to clover cyst nematode ( Heterodera trifolii ) has been successfully transferred from Trifolium nigrescens to T. repens by interspecific hybridisation. A sterile triploid hybrid (H-6909-5, 2 n =3 x =24) was initially produced with the aid of embryo culture. The hybrid was chromosome-doubled from axillary meristems by an in vitro colchicine method. Three chromosome-doubled plants were obtained, and these showed a marked increase in pollen stainability from 10% in 3 x H-6909-5 to an average of 89% (range 88–91%) in 6 x H-6909-5. T. nigrescens was a source of clover cyst nematode resistance. A mean of 23 (range 0–150) cysts per plant was recorded for T. nigrescens in comparison to a mean of 150 (range 50–240) cysts per plant for T. repens. The 3 x and 6 x interspecific hybrids were shown to be as resistant as the most resistant. T. nigrescens genotype and were significantly lower in cyst number per gram of root dry weight than the susceptible T. nigrescens and T . repens genotypes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png TAG Theoretical and Applied Genetics Springer Journals

Transfer of clover cyst nematode resistance from Trifolium nigrescens Viv. to T. repens L. by interspecific hybridisation

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References (39)

Publisher
Springer Journals
Copyright
Copyright © 1997 by Springer-Verlag Berlin Heidelberg
Subject
Legacy
ISSN
0040-5752
eISSN
1432-2242
DOI
10.1007/s001220050693
Publisher site
See Article on Publisher Site

Abstract

Resistance to clover cyst nematode ( Heterodera trifolii ) has been successfully transferred from Trifolium nigrescens to T. repens by interspecific hybridisation. A sterile triploid hybrid (H-6909-5, 2 n =3 x =24) was initially produced with the aid of embryo culture. The hybrid was chromosome-doubled from axillary meristems by an in vitro colchicine method. Three chromosome-doubled plants were obtained, and these showed a marked increase in pollen stainability from 10% in 3 x H-6909-5 to an average of 89% (range 88–91%) in 6 x H-6909-5. T. nigrescens was a source of clover cyst nematode resistance. A mean of 23 (range 0–150) cysts per plant was recorded for T. nigrescens in comparison to a mean of 150 (range 50–240) cysts per plant for T. repens. The 3 x and 6 x interspecific hybrids were shown to be as resistant as the most resistant. T. nigrescens genotype and were significantly lower in cyst number per gram of root dry weight than the susceptible T. nigrescens and T . repens genotypes.

Journal

TAG Theoretical and Applied GeneticsSpringer Journals

Published: Dec 1, 1997

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