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Voltage-gated ion channels in central neurons of Helicoverpa armigera as potential targets for cycloxaprid: a cis-configuration neonicotinoid insecticide

Voltage-gated ion channels in central neurons of Helicoverpa armigera as potential targets for... Cycloxaprid is a novel cis-neonicotinoid, mainly acting on the nicotinic acetylcholine receptor; however, it is not clear whether cycloxaprid can act on voltage-gated ion channels. In this study, the effects of cycloxaprid on the sodium, calcium and potassium channels in central neurons acutely dissociated from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were investigated by the whole-cell patch clamp technique. With the application of cycloxaprid, the half voltage (V0.5) of activation and inactivation of sodium channels exhibited an obvious hyperpolarizing shift around 4–16 mV and 4–14 mV, respectively. The window currents of sodium channels increased by 35.04–88.89%. The time course of recovery from inactivation was also significantly prolonged by 0.25–0.43 ms. The V0.5 of activation and inactivation of calcium channels exhibited a marked hyperpolarizing shift around 6–9 mV and 13–19 mV, respectively. The window currents of calcium channels increased by 13.82–28.97%. The time course of recovery from inactivation for calcium channels was prolonged by 0.76–16.85 ms, although not significantly. Comparatively, the peak currents and the V0.5 of activation of potassium channels showed no significant change. These results indicate that sodium and calcium channels of H. armigera are potential target sites of cycloxaprid. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied Entomology and Zoology Springer Journals

Voltage-gated ion channels in central neurons of Helicoverpa armigera as potential targets for cycloxaprid: a cis-configuration neonicotinoid insecticide

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References (82)

Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to The Japanese Society of Applied Entomology and Zoology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
0003-6862
eISSN
1347-605X
DOI
10.1007/s13355-023-00816-x
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Abstract

Cycloxaprid is a novel cis-neonicotinoid, mainly acting on the nicotinic acetylcholine receptor; however, it is not clear whether cycloxaprid can act on voltage-gated ion channels. In this study, the effects of cycloxaprid on the sodium, calcium and potassium channels in central neurons acutely dissociated from Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were investigated by the whole-cell patch clamp technique. With the application of cycloxaprid, the half voltage (V0.5) of activation and inactivation of sodium channels exhibited an obvious hyperpolarizing shift around 4–16 mV and 4–14 mV, respectively. The window currents of sodium channels increased by 35.04–88.89%. The time course of recovery from inactivation was also significantly prolonged by 0.25–0.43 ms. The V0.5 of activation and inactivation of calcium channels exhibited a marked hyperpolarizing shift around 6–9 mV and 13–19 mV, respectively. The window currents of calcium channels increased by 13.82–28.97%. The time course of recovery from inactivation for calcium channels was prolonged by 0.76–16.85 ms, although not significantly. Comparatively, the peak currents and the V0.5 of activation of potassium channels showed no significant change. These results indicate that sodium and calcium channels of H. armigera are potential target sites of cycloxaprid.

Journal

Applied Entomology and Zoology – Springer Journals

Published: May 1, 2023

Keywords: Cycloxaprid; Helicoverpa armigera; Voltage-gated calcium channel; Voltage-gated potassium channel; Voltage-gated sodium channel

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