Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Zinc and Manganese Imbalances in BALB/c Mice Experimentally Infected with Leishmania (Leishmania) amazonensis

Zinc and Manganese Imbalances in BALB/c Mice Experimentally Infected with Leishmania (Leishmania)... PurposeThe clinical progression of Leishmania (Leishmania) amazonensis infection depends on multiple factors, including immunological status of the host and their genotypic interaction. Several immunological processes depend directly on minerals for an efficient performance. Therefore, this study used an experimental model to investigate the alterations of trace metals in L. amazonensis infection associate with clinical outcome, parasite load, and histopathological lesions, and the effect of CD4 + T cells depletion on these parameters.MethodsA total of 28 BALB/c mice were divided into 4 groups: 1—non-infected; 2—treated with anti-CD4 antibody; 3—infected with L. amazonensis; and 4—treated with anti-CD4 antibody and infected with L. amazonensis. After 24 weeks post-infection, levels of calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), Cu, and Zn were determined by inductively coupled plasma optical emission spectroscopy using tissue samples of the spleen, liver, and kidneys. Additionally, parasite burdens were determined in the infected footpad (inoculation site) and samples of inguinal lymph node, spleen, liver, and kidneys were submitted to histopathological analysis.ResultsDespite no significant difference was observed between groups 3 and 4, L. amazonensis-infected mice had a significant reduction of Zn (65.68–68.32%) and Mn (65.98 to 82.17%) levels. Presence of L. amazonensis amastigotes was also detected in the inguinal lymph node, spleen, and liver samples in all infected animals.ConclusionThe results showed that significant alterations in micro-elements levels occur in BALB/c mice experimentally infected with L. amazonensis and may increase the susceptibility of individuals to the infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Parasitologica Springer Journals

Zinc and Manganese Imbalances in BALB/c Mice Experimentally Infected with Leishmania (Leishmania) amazonensis

Download PDF
6 pages

Loading next page...
 
/lp/springer-journals/zinc-and-manganese-imbalances-in-balb-c-mice-experimentally-infected-Qk5NL9stOP
Publisher
Springer Journals
Copyright
Copyright © The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2023
ISSN
1230-2821
eISSN
1896-1851
DOI
10.1007/s11686-023-00666-1
Publisher site
See Article on Publisher Site

Abstract

PurposeThe clinical progression of Leishmania (Leishmania) amazonensis infection depends on multiple factors, including immunological status of the host and their genotypic interaction. Several immunological processes depend directly on minerals for an efficient performance. Therefore, this study used an experimental model to investigate the alterations of trace metals in L. amazonensis infection associate with clinical outcome, parasite load, and histopathological lesions, and the effect of CD4 + T cells depletion on these parameters.MethodsA total of 28 BALB/c mice were divided into 4 groups: 1—non-infected; 2—treated with anti-CD4 antibody; 3—infected with L. amazonensis; and 4—treated with anti-CD4 antibody and infected with L. amazonensis. After 24 weeks post-infection, levels of calcium (Ca), iron (Fe), magnesium (Mg), manganese (Mn), Cu, and Zn were determined by inductively coupled plasma optical emission spectroscopy using tissue samples of the spleen, liver, and kidneys. Additionally, parasite burdens were determined in the infected footpad (inoculation site) and samples of inguinal lymph node, spleen, liver, and kidneys were submitted to histopathological analysis.ResultsDespite no significant difference was observed between groups 3 and 4, L. amazonensis-infected mice had a significant reduction of Zn (65.68–68.32%) and Mn (65.98 to 82.17%) levels. Presence of L. amazonensis amastigotes was also detected in the inguinal lymph node, spleen, and liver samples in all infected animals.ConclusionThe results showed that significant alterations in micro-elements levels occur in BALB/c mice experimentally infected with L. amazonensis and may increase the susceptibility of individuals to the infection.

Journal

Acta Parasitologica – Springer Journals

Published: Mar 8, 2023

Keywords: Leishmania sp.; Cutaneous leishmaniasis; Microelements; Trace elements; CD4 + T cells

References

  • Identification and distribution of new world Leishmania species characterized by Serodeme analysis using monoclonal antibodies
    Grimaldi, G; David, JR; McMahon-Pratt, D
  • Leishmania amazonensis infections in Oryzomys acritus and Oryzomys nitidus from Bolivia
    Kerr, SF; Emmons, LH; Melby, PC; Liu, C; Perez, LE; Villegas, M; Miranda, R
  • Molecular characterization and geographical distribution of leishmaniasis aethiological agents in Peru
    Urbano, J; Minaya, G; Sa Nchez-Moreno, M; Sánchez, RG; Marín, C
  • First molecular report of Leishmania (Leishmania) amazonensis and Leishmania (Viannia) guyanensis in Paraguayan Inhabitants Using High-Resolution Melt-PCR
    Salvioni Recalde, OD; Pereira Brunelli, J; Rolon, MS; Rojas de Arias, A; Aldama, O; Gómez, CV
  • Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP
    Simon, S; Nacher, M; Carme, B; Basurko, C; Roger, A; Adenis, A; Ginouves, M; Demar, M; Couppie, P
  • Taxonomy, diversity, temporal and geographical distribution of Cutaneous Leishmaniasis in Colombia: a retrospective study
    Ramírez, JD; Hernández, C; León, CM; Ayala, MS; Flórez, C; González, C
  • Epidemiology and ecology of leishmaniasis in Latin-America
    Lainson, R; Shaw, J
  • Leishmania in synanthropic rodents (Rattus rattus): new evidence for the urbanization of Leishmania (Leishmania) amazonensis
    Caldart, ET; Freire, RL; Ferreira, FP; Ruffolo, BB; Sbeghen, MR; Mareze, M; Garcia, JL; Mitsuka-Breganó, R; Navarro, IT
  • Detection of Leishmania (Leishmania) amazonensis and Leishmania (Leishmania) infantum chagasi in Brazilian bats
    Savani, ESMM; Almeida, MF; Oliveira Camargo, MCG; D’Auria, SRN; Silva, MMS; Oliveira, ML; Sacramento, D
  • Feline leishmaniasis due to Leishmania (Leishmania) amazonensis in Mato Grosso do Sul State
    Souza, AI; Barros, EMS; Ishikawa, E; Ilha, IMN; Marin, GRB; Nunes, VLB
  • The first records of Leishmania (Leishmania) amazonensis in dogs (Canis familiaris) diagnosed clinically as having canine visceral leishmaniasis from Araçatuba County, São Paulo State
    Tolezano, JE; Uliana, SRB; Taniguchi, HH; Araújo, MFL; Barbosa, JAR; Barbosa, JER; Floeter-Winter, LM; Shaw, JJ
  • Canine leishmaniasis: an overview of the current status and strategies for control
    Ribeiro, RR; Michalick, MSM; da Silva, ME; dos Santos, CCP; Frézard, FJG; da Silva, SM
  • Immunological characteristics of experimental murine infection with Leishmania (Leishmania) amazonensis
    Pereira, BAS; Alves, CR
  • New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes
    Chvapil, M
  • Changes in the total content of iron, copper, and zinc in serum, heart, liver, spleen, and skeletal muscle tissues of rats infected with Trypanosoma cruzi
    de Abel, M; de la Cruz, AJ; Burguera, JL; Burguera, M; Añez, N
  • Effect of high dietary zinc on plasma ceruloplasmin and erythrocyte superoxide dismutase activities in copper-depleted and repleted rats
    Panemangalore, M; Bebe, FN
  • Zinc-controlled Th1/Th2 switch significantly determines development of diseases
    Sprietsma, J
  • Alterations of serum selenium, zinc, copper, and iron concentrations and some related antioxidant enzyme activities in patients with cutaneous leishmaniasis
    Kocyigit, A; Erel, O; Gurel, MS; Avci, S; Aktepe, N
  • Effects of antimonial therapy on serum zinc, copper and iron concentrations in patients with cutaneous leishmaniasis in Turkey
    Kocyigit, A; Erel, Ö; Seyrek, A; Gürel, M; Aktepe, N; Avci, S; Vural, H
  • Biochemical association between essential trace elements and susceptibility to Leishmania major in BALB/c and C57BL/6 mice
    Amini, M; Nahrevanian, H; Khatami, S; Farahmand, M; Mirkhani, F; Javadian, S
  • Alterations of serum zinc, copper and iron concentrations in patients with acute and chronic cutaneous leishmaniasis
    Faryadi, M; Mohebali, M
  • Glutathione and the redox control system trypanothione/trypanothione reductase are involved in the protection of Leishmania spp against nitrosothiol-induced cytotoxicity
    Romão, P; Tovar, J; Fonseca, S; Moraes, R; Cruz, A; Hothersall, J; Noronha-Dutra, AA; Ferreira, SH; Cunha, FQ
  • Culture microtitration: a sensitive method for quantifying Leishmania infantum in tissues of infected mice
    Buffet, P; Sulahian, A; Garin, Y; Nassar, N; Derouin, F
  • A method for the combined staining of cells and fibers in the nervous system
    Klüver, H; Barrera, E
  • Serum and hair levels of zinc and other elements in dogs with visceral leishmaniasis
    Pasa, S; Kargin, F; Bildik, A; Seyrek, K; Ozbel, Y; Ozensoy, S
  • Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
    Van Weyenbergh, J; Santana, G; D'Oliveira, A; Santos, AF; Costa, CH; Carvalho, EM; Barral, A; Barral-Netto, M
  • A potential link among antioxidant enzymes, histopathology and trace elements in canine visceral leishmaniasis
    Souza, CC; Barreto, TO; da Silva, SM; Pinto, AWJ; Figueiredo, MM; Ferreira Rocha, OG; Cangussú, SD; Tafuri, WL
  • Canine visceral leishmaniasis: relationships between oxidative stress, liver and kidney variables, trace elements, and clinical status
    Heidarpour, M; Soltani, S; Mohri, M; Khoshnegah, J
  • The effect of metals on IL-2-related lymphocyte proliferation
    Warner, GL; Lawrence, DA
  • Trace metal, acute phase and metabolic response to endotoxin in metallothionein-null mice
    Rofe, AM; Philcox, JC; Coyle, P
  • Experimental mixed infection of Leishmania (Leishmania) amazonensis and Leishmania (L.) infantum in hamsters (Mesocricetus auratus)
    De Lima Celeste, JL; Venuto Moura, AP; FranÇA-Silva, JC; Matos De Sousa, G; Oliveira Silva, S; Norma Melo, M; Luiz Tafuri, W; Carvalho Souza, C; Andrade, MDE; H,
  • Associations among plasma selenium, zinc, copper, and iron concentrations and immunoregulatory cytokine levels in patients with cutaneous leishmaniasis
    Kocyigit, A; Gur, S; Erel, Ö; Gürel, MS
  • Evaluation of serum mineral status and hormone profile in goats and some of their inter-relations
    Yatoo, MI; Saxena, A; Kumar, P; Gugjoo, MB; Dimri, U; Sharma, MC; Jhambh, R
  • Atypical American visceral leishmaniasis caused by disseminated Leishmania amazonensis infection presenting with hepatitis and adenopathy
    Aleixo, JA; Nascimento, ET; Monteiro, GR; Fernandes, MZ; Ramos, AMO; Wilson, ME; Pearson, RD; Jeronimo, SM
  • Eco-epidemiology of visceral leishmaniasis in the urban area of Paracatu, state of Minas Gerais
    Dias, ES; Regina-Silva, S; França-Silva, JC; Paz, GF; Michalsky, ÉM; Araújo, SC; Valadão, JL; de Oliveira, L-S; de Oliveira, FS; Pacheco, RS; Fortes-Dias, CL
  • Comparative genomics of canine-isolated Leishmania (Leishmania) amazonensis from an endemic focus of visceral leishmaniasis in Governador Valadares, southeastern Brazil
    Valdivia, HO; Almeida, LV; Roatt, BM; Reis-Cunha, JL; Pereira, AAS; Gontijo, C; Fujiwara, RT; Reis, AB; Sanders, MJ; Cotton, JA; Bartholomeu, DC
  • Natural canine infection by Leishmania infantum and Leishmania amazonensis and their implications for disease control
    Sanches, LdC; Martini, CCd; Nakamura, AA; Santiago, MEB; Dolabela de Lima, B; Lima, VMF
  • Clinical and pathological aspects of canine cutaneous Leishmaniasis: a meta-analysis
    Oliveira, CS; Ratzlaff, FR; Pötter, L; Romão, PRT; Botton, SdA; Vogel, FSF; Sangioni, LA