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A Microextraction Coupled with HPLC-UV for a Sensitive Detection of Estrogens in Water

A Microextraction Coupled with HPLC-UV for a Sensitive Detection of Estrogens in Water AbstractEnvironmental concerns over trace levels of estrogens concentrations in water environment have increased because of their adverse effects on humans, animals and aquatic life. In this contribution, we developed a simple and highly sensitive analytical protocol for trace analysis of 3 important estrogens namely 17α-ethinylestradiol (EE2), 17β-estradiol (E2), and diethylstilbestrol (DES) in water, resulting in pg/mL level detection. Microextraction by packed sorbent (MEPS) using a C18 sorbent in combination with HPLC-UV was applied to extract/enrich, separate, identify and quantify the estrogens at pg/mL level in water. Achieved recoveries in spiked water samples ranged from 85.02 to 98.07 % with RSD (%) ranged from 5.11–9.12. Limit of detection (LODs) were as low as 0.09–0.47 pg/mL in spiked samples. To investigate the real world application, the protocol was applied to a total of 9 real water samples including drinking water, private well water, hospital waste water, and river water, and yielded concentrations ranging for two estrogens (EE2 and E2) from 2.21 to 3.75 ng/mL, and DES was not found in any real sample studied. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

A Microextraction Coupled with HPLC-UV for a Sensitive Detection of Estrogens in Water

Saini, Shivender Singh
Analytical Chemistry Letters , Volume 8 (5): 16 – Sep 3, 2018
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A Microextraction Coupled with HPLC-UV for a Sensitive Detection of Estrogens in Water

Abstract

AbstractEnvironmental concerns over trace levels of estrogens concentrations in water environment have increased because of their adverse effects on humans, animals and aquatic life. In this contribution, we developed a simple and highly sensitive analytical protocol for trace analysis of 3 important estrogens namely 17α-ethinylestradiol (EE2), 17β-estradiol (E2), and diethylstilbestrol (DES) in water, resulting in pg/mL level detection. Microextraction by packed sorbent (MEPS)...
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Publisher
Taylor & Francis
Copyright
© 2018 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2018.1498017
Publisher site
See Article on Publisher Site

Abstract

AbstractEnvironmental concerns over trace levels of estrogens concentrations in water environment have increased because of their adverse effects on humans, animals and aquatic life. In this contribution, we developed a simple and highly sensitive analytical protocol for trace analysis of 3 important estrogens namely 17α-ethinylestradiol (EE2), 17β-estradiol (E2), and diethylstilbestrol (DES) in water, resulting in pg/mL level detection. Microextraction by packed sorbent (MEPS) using a C18 sorbent in combination with HPLC-UV was applied to extract/enrich, separate, identify and quantify the estrogens at pg/mL level in water. Achieved recoveries in spiked water samples ranged from 85.02 to 98.07 % with RSD (%) ranged from 5.11–9.12. Limit of detection (LODs) were as low as 0.09–0.47 pg/mL in spiked samples. To investigate the real world application, the protocol was applied to a total of 9 real water samples including drinking water, private well water, hospital waste water, and river water, and yielded concentrations ranging for two estrogens (EE2 and E2) from 2.21 to 3.75 ng/mL, and DES was not found in any real sample studied.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Sep 3, 2018

Keywords: 17α-Ethinylestradiol (EE2); 17β-estradiol (E2); diethylstilbestrol (DES); trace; microextraction by packed sorbent (MEPS); HPLC-UV; water

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