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Analysis of Chloramphenicol in Biological Samples by SPE-HPLC

Analysis of Chloramphenicol in Biological Samples by SPE-HPLC Abstract: Chloramphenicol (CAP) is a widely used antibiotic for bacterial diseases for animals and in typhoid fever in human beings. SPE-HPLC method is described for the detection of chloramphenicol from human blood plasma samples. Frusemide (FRU) was taken as an internal standard to determine the percentage recovery of chloramphenicol in blood sample. Their standard solutions were prepared in methanol having the concentration of 1.0 mg/mL. The extraction of both the drugs was carried out by SPE using methanol at a flow rate of 0.1mL/min. Further, the analysis was done by HPLC having monolithic, chromolith performance RP-18e, 100-4.6 (100 × 4.6 mm) column. Phosphate buffer (100 mM, pH 2.5)-acetonitrile (75:25, v/v) was selected as a mobile phase at a flow rate of 1.5 mL/min. Detection of the drugs were carried out at 270 nm. The percentage recoveries of CAP and FRU from plasma samples were 92.0 and 90.0, respectively. The detection limit of the developed method for CAP and FRU were 0.8 µg/mL and 1.0 µg/mL, respectively. The result of separation factor (α) and resolution factor (Rs) of CAP with respect to FRU in plasma samples were 2.31 and 16.25, respectively. The developed method may be used for quantification and detection of CAP from samples of biological origin. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Analysis of Chloramphenicol in Biological Samples by SPE-HPLC

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Analysis of Chloramphenicol in Biological Samples by SPE-HPLC

Abstract

Abstract: Chloramphenicol (CAP) is a widely used antibiotic for bacterial diseases for animals and in typhoid fever in human beings. SPE-HPLC method is described for the detection of chloramphenicol from human blood plasma samples. Frusemide (FRU) was taken as an internal standard to determine the percentage recovery of chloramphenicol in blood sample. Their standard solutions were prepared in methanol having the concentration of 1.0 mg/mL. The extraction of both the drugs was carried...
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Publisher
Taylor & Francis
Copyright
© 2013 The Experimental Psychology Society
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2013.806413
Publisher site
See Article on Publisher Site

Abstract

Abstract: Chloramphenicol (CAP) is a widely used antibiotic for bacterial diseases for animals and in typhoid fever in human beings. SPE-HPLC method is described for the detection of chloramphenicol from human blood plasma samples. Frusemide (FRU) was taken as an internal standard to determine the percentage recovery of chloramphenicol in blood sample. Their standard solutions were prepared in methanol having the concentration of 1.0 mg/mL. The extraction of both the drugs was carried out by SPE using methanol at a flow rate of 0.1mL/min. Further, the analysis was done by HPLC having monolithic, chromolith performance RP-18e, 100-4.6 (100 × 4.6 mm) column. Phosphate buffer (100 mM, pH 2.5)-acetonitrile (75:25, v/v) was selected as a mobile phase at a flow rate of 1.5 mL/min. Detection of the drugs were carried out at 270 nm. The percentage recoveries of CAP and FRU from plasma samples were 92.0 and 90.0, respectively. The detection limit of the developed method for CAP and FRU were 0.8 µg/mL and 1.0 µg/mL, respectively. The result of separation factor (α) and resolution factor (Rs) of CAP with respect to FRU in plasma samples were 2.31 and 16.25, respectively. The developed method may be used for quantification and detection of CAP from samples of biological origin.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: May 4, 2013

Keywords: Chloramphenicol; biological samples; frusemide; SPE; HPLC

References

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