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Comparative Study of the Resolution Efficiency of High-performance Liquid Chromatographic and Chemometrics-assisted UV Spectrophotometric Methods: Application on Pharmaceutical Mixtures

Comparative Study of the Resolution Efficiency of High-performance Liquid Chromatographic and... AbstractHigh-performance liquid chromatographic and two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of pharmaceutical binary mixtures of lidocaine hydrochloride(LH) and fluocortolone pivalate, (FCP) and their ternary mixture with chlorquinaldol (CQ) without any chemical pretreatment. The chromatographic separation was achieved on normal-phase C8 analytical column with a mobile phase consisting of a mixture of phosphate buffer : acetonitrile (40:60, v/v) buffered with 0.5% triethylamine, (adjusted to pH 3.3 ± 0.2 with o- phosphoric acid) with flow rate 1 mL/min. Quantitation was achieved with UV detection at 220 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture. The proposed methods were validated and successfully applied for the analysis of dosage forms and laboratory-prepared mixtures containing the components combination. The developed analytical methods did not show any interference of the excipients when applied to pharmaceutical dosage forms. The results of HPLC were compared with PLS-1 and PCR methods and a good agreement was found. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Comparative Study of the Resolution Efficiency of High-performance Liquid Chromatographic and Chemometrics-assisted UV Spectrophotometric Methods: Application on Pharmaceutical Mixtures

Comparative Study of the Resolution Efficiency of High-performance Liquid Chromatographic and Chemometrics-assisted UV Spectrophotometric Methods: Application on Pharmaceutical Mixtures

Abstract

AbstractHigh-performance liquid chromatographic and two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of pharmaceutical binary mixtures of lidocaine hydrochloride(LH) and fluocortolone pivalate, (FCP) and their ternary mixture with chlorquinaldol (CQ) without any chemical pretreatment. The chromatographic separation was achieved on normal-phase C8 analytical column with a mobile phase consisting of a mixture of phosphate buffer : acetonitrile (40:60,...
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Publisher
Taylor & Francis
Copyright
© 2016 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2016.1172505
Publisher site
See Article on Publisher Site

Abstract

AbstractHigh-performance liquid chromatographic and two chemometrics-assisted UV spectrophotometric methods were proposed for the resolution of pharmaceutical binary mixtures of lidocaine hydrochloride(LH) and fluocortolone pivalate, (FCP) and their ternary mixture with chlorquinaldol (CQ) without any chemical pretreatment. The chromatographic separation was achieved on normal-phase C8 analytical column with a mobile phase consisting of a mixture of phosphate buffer : acetonitrile (40:60, v/v) buffered with 0.5% triethylamine, (adjusted to pH 3.3 ± 0.2 with o- phosphoric acid) with flow rate 1 mL/min. Quantitation was achieved with UV detection at 220 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture. The proposed methods were validated and successfully applied for the analysis of dosage forms and laboratory-prepared mixtures containing the components combination. The developed analytical methods did not show any interference of the excipients when applied to pharmaceutical dosage forms. The results of HPLC were compared with PLS-1 and PCR methods and a good agreement was found.

Journal

Analytical Chemistry LettersTaylor & Francis

Published: May 3, 2016

Keywords: Lidocaine hydrochloride; Fluocortolone Pivalate; Chlorquinaldol; HPLC; chemo- metrics-assisted spectrophotometry

References