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Determination of Itraconazole in Bulk and Human Plasma by High Performance Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometry

Determination of Itraconazole in Bulk and Human Plasma by High Performance Liquid... AbstractA fast, sensitive and selective high performance liquid chromatography-electron spray ionization-tandem mass spectrometry method (HPLC-ESI-MS/MS) was produced and validated for the determination of itraconazole (ITC) in bulk and human plasma on an intersil, ODS-3, C18 column using a mobile phase consisting of acetonitrile: ammonium formate (80:20, v/v)(pH 3.5) with flow rate 0.5 ml/min. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode utilizing m/z 705.3 → m/z 392.3 for ITC and m/z 559.3 → m/z 440.3 for atorvastatin as an internal standard (IS). Different parameters were discussed, e.g: type of columns, mobile phase pH, mobile phase composition, rate of fragmentation, and rate of collision. .The mass detection was performed using electrospray ionization in the positive mode (ESI +). The method was linear over the concentration range 4- 1000 ng/ml for ITC with r2 = 0.99 . The limit of detection and limit of quantification were 1.17 and 3.54 ng/ml in human plasma, respectively. Intra-and between day accuracy demonstrated % RSD < 1.50%. The outcome acquired by the proposed strategy was contrasted and that got by the reference LC-MS method. The technique demonstrated great selectivity, repeatability, linearity and affectability as indicated by the assessment of the validation parameters. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Determination of Itraconazole in Bulk and Human Plasma by High Performance Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometry

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Determination of Itraconazole in Bulk and Human Plasma by High Performance Liquid Chromatographic-Electrospray Ionization-Tandem Mass Spectrometry

Abstract

AbstractA fast, sensitive and selective high performance liquid chromatography-electron spray ionization-tandem mass spectrometry method (HPLC-ESI-MS/MS) was produced and validated for the determination of itraconazole (ITC) in bulk and human plasma on an intersil, ODS-3, C18 column using a mobile phase consisting of acetonitrile: ammonium formate (80:20, v/v)(pH 3.5) with flow rate 0.5 ml/min. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction...
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Publisher
Taylor & Francis
Copyright
© 2016 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2016.1247749
Publisher site
See Article on Publisher Site

Abstract

AbstractA fast, sensitive and selective high performance liquid chromatography-electron spray ionization-tandem mass spectrometry method (HPLC-ESI-MS/MS) was produced and validated for the determination of itraconazole (ITC) in bulk and human plasma on an intersil, ODS-3, C18 column using a mobile phase consisting of acetonitrile: ammonium formate (80:20, v/v)(pH 3.5) with flow rate 0.5 ml/min. Detection was carried out on a triple quadrupole tandem mass spectrometer in multiple reaction monitoring (MRM) mode utilizing m/z 705.3 → m/z 392.3 for ITC and m/z 559.3 → m/z 440.3 for atorvastatin as an internal standard (IS). Different parameters were discussed, e.g: type of columns, mobile phase pH, mobile phase composition, rate of fragmentation, and rate of collision. .The mass detection was performed using electrospray ionization in the positive mode (ESI +). The method was linear over the concentration range 4- 1000 ng/ml for ITC with r2 = 0.99 . The limit of detection and limit of quantification were 1.17 and 3.54 ng/ml in human plasma, respectively. Intra-and between day accuracy demonstrated % RSD < 1.50%. The outcome acquired by the proposed strategy was contrasted and that got by the reference LC-MS method. The technique demonstrated great selectivity, repeatability, linearity and affectability as indicated by the assessment of the validation parameters.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Nov 1, 2016

Keywords: Itraconazole; liquid chromatographic-tandem mass spectrometry; multiple reaction monitoring; method validation; biomedical analysis

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