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- Publisher
- Taylor & Francis
- Copyright
- © 1969 Société entomologique de France
- ISSN
- 2168-6351
- eISSN
- 0037-9271
- DOI
- 10.1080/21686351.1969.12278919
- Publisher site
- See Article on Publisher Site
Abstract
Summary The black chlorazol E is precious in Entomology on the one hand for the staining of the cuticula, on the other hand for the staining of the tissues of whole small organs. 1) Cuticular staining. Thanks to its stability, in aqueous KOH solution, even warm, black chlorazol allows the simultaneous cleaning and staining of minute pieces, which become so transparent and colourless with KOH only that they risk to be lost. Black chlorazol sets mainly on the endocuticula and the intima lining the cavity of ectodermic organs connected to the integument. By applying it in the form of a stream of alcoholic solution aimed at the object to be prepared, immersed in lactophenol, it is possible to stain selectively some elements of the endoskeleton or the internal mould of certain organs. 2) Staining of whole organs. The interest, already known, of black chlorazol for showing off various histological elements, is much increased by the possibility to differentiate the staining. This differentiation, only in the case of whole organs, is done with a concentrated aqueous solution of chloral hydrate. Black chlorazol shows a remarkable tinctorial affinity, which until now had not been pointed out, for the spermatozoa.
Journal
Annales de la Société entomologique de France (N S ) – Taylor & Francis
Published: Jan 31, 1969