Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

In vitro Studies of Interaction Between Dexamethasone and NSAIDS by Using RP-High Performance Liquid Chromatography

In vitro Studies of Interaction Between Dexamethasone and NSAIDS by Using RP-High Performance... AbstractNon-steroidal anti-inflammatory drugs (NSAIDS) are commonly used because of their remarkable cure over mild and moderate pain including osteoarthritis and rheumatoid arthritis. Medical doctors commonly prescribe steroidal drugs in combination with the NSAID’s therefore we have developed a method to evaluate the percent availability of these two classes of drugs and analyzed their possible interactions. The proposed method is validated according to the International Conference on Harmonization (ICH) guide lines on RP-HPLC (Reverse Phase High Performance Liquid Chromatography). A good chromatographic separation was achieved using a mobile phase containing methanol-water (80:20 v/v) at pH = 3.2 with a flow rate of 1.0 mL/min (detection at 265 nm with a UV-vis detector). The in-vitro interaction study was conducted at physiological conditions (i.e. temperature 37°C and stomach pH = 3.2). The method provided a very good percent recovery of different molecules. The percent recovery of Piroxicam, Naproxen sodium, Flurbiprofen and Ibuprofen are 100.539, 100.373, 99.683 and 100.840 respectively. The results indicated that percent availability of all NSAIDs and dexamethasone reduced during the time interval 2.5 h which is likely due to the formation of charge transfer complexes. We can conclude that simultaneous administration of these drugs may alter their bio-availability. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

In vitro Studies of Interaction Between Dexamethasone and NSAIDS by Using RP-High Performance Liquid Chromatography

Download PDF

In vitro Studies of Interaction Between Dexamethasone and NSAIDS by Using RP-High Performance Liquid Chromatography

Abstract

AbstractNon-steroidal anti-inflammatory drugs (NSAIDS) are commonly used because of their remarkable cure over mild and moderate pain including osteoarthritis and rheumatoid arthritis. Medical doctors commonly prescribe steroidal drugs in combination with the NSAID’s therefore we have developed a method to evaluate the percent availability of these two classes of drugs and analyzed their possible interactions. The proposed method is validated according to the International Conference on...
Loading next page...
 
/lp/taylor-francis/in-vitro-studies-of-interaction-between-dexamethasone-and-nsaids-by-gR46NMdjj6
Publisher
Taylor & Francis
Copyright
© 2018 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2018.1426491
Publisher site
See Article on Publisher Site

Abstract

AbstractNon-steroidal anti-inflammatory drugs (NSAIDS) are commonly used because of their remarkable cure over mild and moderate pain including osteoarthritis and rheumatoid arthritis. Medical doctors commonly prescribe steroidal drugs in combination with the NSAID’s therefore we have developed a method to evaluate the percent availability of these two classes of drugs and analyzed their possible interactions. The proposed method is validated according to the International Conference on Harmonization (ICH) guide lines on RP-HPLC (Reverse Phase High Performance Liquid Chromatography). A good chromatographic separation was achieved using a mobile phase containing methanol-water (80:20 v/v) at pH = 3.2 with a flow rate of 1.0 mL/min (detection at 265 nm with a UV-vis detector). The in-vitro interaction study was conducted at physiological conditions (i.e. temperature 37°C and stomach pH = 3.2). The method provided a very good percent recovery of different molecules. The percent recovery of Piroxicam, Naproxen sodium, Flurbiprofen and Ibuprofen are 100.539, 100.373, 99.683 and 100.840 respectively. The results indicated that percent availability of all NSAIDs and dexamethasone reduced during the time interval 2.5 h which is likely due to the formation of charge transfer complexes. We can conclude that simultaneous administration of these drugs may alter their bio-availability.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Jan 2, 2018

Keywords: NSAIDs; Dexamethasone; RP-HPLC; Interaction Studies

References

  • Sample pretreatment and determination of non steroidal anti-inflammatory drugs (NSAIDs) in pharmaceutical formulations and biological samples (blood, plasma, erythrocytes) by HPLC-UV-MS and ì-HPLC
  • Simultaneous determination of non-steroidal anti-inflammatory Drugs in Pharmaceutical formulations and Human Serum by Reversed Phase High Performance Liquid Chromatography
  • Simultaneous analysis of several non-steroidal anti-inflammatory drugs in human urine by high-performance liquid chromatography with normal solid-phase extraction
  • Adverse drug reactions associated with the use of NSAIDs: A case/noncase analysis of spontaneous reports from the French pharmacovigilance database 2002–2006
  • Chiral separation and modeling of baclofen, bupropion, and etodolac profens on amylose reversed phase chiral column
  • Development and validation of SPMMTE HPLC method for analysis of profens from human plasma
  • Analysis of ibuprofen, pantoprazole and itopride combination therapeutic drugs in human plasma by solid phase membrane microtip extraction and high-performance liquid chromatography methods using new generation core shell C18 column
  • Synthesis of composite iron nano adsorbent and removal of ibuprofen drug residue from water
  • Enantiomeric resolution of Ibuprofen and Flurbiprofen in human plasma by SPE-Chiral HPLC Methods
  • Toxicopathological overview of analgesic and anti-inflammatory drugs in animals
  • Chiral Analysis of Ibuprofen Residues in Water and Sediment
  • Supramolecular Chiro-Biomedical Assays and Enantioselective. HPLC Analyses for Evaluation of Profens as Non-Steroidal Anti-Inflammatory Drugs
  • Optimization of the chiral resolution of baclofen by capillary electrophoresis using β-cyclodextrin as the chiral selector
  • Mitigating the cardiovascular and renal effects of NSAIDs
  • A review of Analytical techniques for Determination of Oxicams, Nimesulide and Nabumetone
  • Drug interactions-principles, examples and clinical consequences
  • Interaction between NSAIDs and steroid in rat stomach
  • Steroids and risk of upper gastrointestinal complications
  • Simultaneous determination of dexamethasone and trimethoprim by liquid chromatography
  • Determination of dexamethasone acetate in cream by HPLC
  • Simultaneous Determination of Dexamethasone and Moxifloxacin in Pharmaceutical formulations using stability indicating HPLC method
  • High throughput HPLC-ESI-MS method for the quantitation of Dexamethasone in blood plasma
  • Dexamethasone fails to produce antipyretic and analgesic actions in experimental animals
  • Drug Interaction Studies of Gliquidone with Fexofenadine, Cetirizine, and Levocetirizine
  • Drug-drug interaction between Diclofenac, Cetirizine and Ranitidine
  • Spectroscopic characterization of in vitro interactions of Cetirizine and NSAIDS
  • Detecting drug-drug interactions using a database for spontaneous adverse drug reactions: an example with diuretics and Non-steroidal Anti-inflammatory Drugs
  • Pharmacokinetic drug interactions with Non-steroidal Anti-Inflammatory Drugs
  • In Vitro Studies of Interaction between Metformin and NSAIDS (Non Steroidal Anti-Inflamatory Drugs) using Spectrophotometry and RP-High Performance Liquid Chromatography