Abstract

AbstractA phytochemical screening revealed the presence of phytoconstituents like flavonoid, anthraquinone and phenol in the callus and suspension culture of Oroxylum indicum (L.) Vent. (Bignoniaceae). The quantitative estimation using TLC technique reflected the presence of two flavonoids viz. baicalein and chrysin in three fractions (chloroform, ethyl acetate and n-butanol) of callus extract and in intra and extracellular suspension cultures. Protocol was developed for quantification of phytoconstituents by HPLC with methanol: water (70:30) mobile phase and c-18 column in all the three fractions of callus extract. Both baicalein and chrysin flavonoids were there in all the three callus fractions. Maximum amount of baicalein was present in nbutanol fraction of callus while maximum amount of chrysin was present in chloroform fraction. Cell suspension cultures of O. indicum were established in shake flask system to optimize medium formulation for cell growth and flavonoid content. Murashige and Skoog’s basal medium (MS) supplemented with 1 mg/L BAP, 0.5 mg /LNAA , 0.1 mg /L 2,4-D and 1 mg /L AgNO3 was found optimum for growth parameters viz. cell counting, PCV, fresh weight and dry weight. Presence of baicalein and chrysin in intracellular and extracellular fractions of this suspension culture is a successful event of present work.