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Quercetin Mediated Inhibition of Hydrogen Peroxide-induced Genomic DNA Damage and Toxicity

Quercetin Mediated Inhibition of Hydrogen Peroxide-induced Genomic DNA Damage and Toxicity AbstractFlavonoids are reported to exhibit a wide variety of biological effects including antioxidant activities. The present study aims to explore the protective effect of the flavonol quercetin against hydrogen peroxide-mediated damage. The effects of quercetin on H2O2-mediated DNA damage were evaluated using spectrophotometric analyses, DNA damage monitoring by gel electrophoresis, cell viability, CBMN assay, comet assay and chromosomal aberration study inhuman peripheral blood lymphocytes. Quercetin was found to be protective at 30 μM in the cell viability study, while it showed its maximum efficacy in the CBMN assay and comet assay at a concentration level of 40 μM against H2O2-induced damage. Spectrophotometric analyses speculated that the intercalation is the possible binding mode of quercetin with DNA and prevented oxidative stress-induced DNA damage. This study successfully establishes quercetin as a protective agent against oxidative DNA damage induced by H2O2. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Biologically Active Products from Nature Taylor & Francis

Quercetin Mediated Inhibition of Hydrogen Peroxide-induced Genomic DNA Damage and Toxicity

Quercetin Mediated Inhibition of Hydrogen Peroxide-induced Genomic DNA Damage and Toxicity

Journal of Biologically Active Products from Nature , Volume 7 (3): 14 – May 4, 2017

Abstract

AbstractFlavonoids are reported to exhibit a wide variety of biological effects including antioxidant activities. The present study aims to explore the protective effect of the flavonol quercetin against hydrogen peroxide-mediated damage. The effects of quercetin on H2O2-mediated DNA damage were evaluated using spectrophotometric analyses, DNA damage monitoring by gel electrophoresis, cell viability, CBMN assay, comet assay and chromosomal aberration study inhuman peripheral blood lymphocytes. Quercetin was found to be protective at 30 μM in the cell viability study, while it showed its maximum efficacy in the CBMN assay and comet assay at a concentration level of 40 μM against H2O2-induced damage. Spectrophotometric analyses speculated that the intercalation is the possible binding mode of quercetin with DNA and prevented oxidative stress-induced DNA damage. This study successfully establishes quercetin as a protective agent against oxidative DNA damage induced by H2O2.

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References (42)

Publisher
Taylor & Francis
Copyright
© 2017 Har Krishan Bhalla & Sons
ISSN
2231-1874
eISSN
2231-1866
DOI
10.1080/22311866.2017.1329665
Publisher site
See Article on Publisher Site

Abstract

AbstractFlavonoids are reported to exhibit a wide variety of biological effects including antioxidant activities. The present study aims to explore the protective effect of the flavonol quercetin against hydrogen peroxide-mediated damage. The effects of quercetin on H2O2-mediated DNA damage were evaluated using spectrophotometric analyses, DNA damage monitoring by gel electrophoresis, cell viability, CBMN assay, comet assay and chromosomal aberration study inhuman peripheral blood lymphocytes. Quercetin was found to be protective at 30 μM in the cell viability study, while it showed its maximum efficacy in the CBMN assay and comet assay at a concentration level of 40 μM against H2O2-induced damage. Spectrophotometric analyses speculated that the intercalation is the possible binding mode of quercetin with DNA and prevented oxidative stress-induced DNA damage. This study successfully establishes quercetin as a protective agent against oxidative DNA damage induced by H2O2.

Journal

Journal of Biologically Active Products from NatureTaylor & Francis

Published: May 4, 2017

Keywords: Hydrogen peroxide; free radicals; DNA damage; calf thymus DNA; CBMN assay

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