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Resolving Problems Encountered in Spectrophotometric Analysis of a Ternary Mixture of Linagliptin, Empagliflozin and Metformin Hydrochloride

Resolving Problems Encountered in Spectrophotometric Analysis of a Ternary Mixture of... Abstract There are two main problems encountered in spectrophotometric analysis of a ternary mixture composed of linagliptin LINA, empagliflozin EMPA and metformin hydrochloride MET. The first problem is due to the severe overlap in their zero and derivative spectra. To overcome this problem, LINA was determined by direct spectrophotometry at λ max = 298 nm where there is no interference from other components while, EMPA and MET were determined using double divisor ratio spectra derivative method DDRD. In this method, the first derivative spectra were calculated for ratio spectra generated by dividing the absorption spectra of ternary mixtures containing increasing concentrations of one of the components by a standard spectrum of binary mixture of the other two components (double divisor). The calibration graphs were constructed by measuring the amplitude at either the minimum or maximum wavelengths against the concentrations. The selected wavelengths for determination of EMPA and MET are 282.92 and 250 nm, respectively. The second problem arises from the presence of LINA and EMPA as minor components in their recently approved dosage form with MET thus, sample enrichment through spiking was employed for LINA and EMPA to enable their spectrophotometric analysis in laboratory prepared mixtures that have the same ratio of the three components as in their pharmaceutical dosage form. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Resolving Problems Encountered in Spectrophotometric Analysis of a Ternary Mixture of Linagliptin, Empagliflozin and Metformin Hydrochloride

Elagamy, Samar H.
Analytical Chemistry Letters , Volume 12 (3): 9 – May 4, 2022
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Resolving Problems Encountered in Spectrophotometric Analysis of a Ternary Mixture of Linagliptin, Empagliflozin and Metformin Hydrochloride

Abstract

Abstract There are two main problems encountered in spectrophotometric analysis of a ternary mixture composed of linagliptin LINA, empagliflozin EMPA and metformin hydrochloride MET. The first problem is due to the severe overlap in their zero and derivative spectra. To overcome this problem, LINA was determined by direct spectrophotometry at λ max = 298 nm where there is no interference from other components while, EMPA and MET were determined using double divisor ratio spectra...
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Publisher
Taylor & Francis
Copyright
© 2022 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2022.2058414
Publisher site
See Article on Publisher Site

Abstract

Abstract There are two main problems encountered in spectrophotometric analysis of a ternary mixture composed of linagliptin LINA, empagliflozin EMPA and metformin hydrochloride MET. The first problem is due to the severe overlap in their zero and derivative spectra. To overcome this problem, LINA was determined by direct spectrophotometry at λ max = 298 nm where there is no interference from other components while, EMPA and MET were determined using double divisor ratio spectra derivative method DDRD. In this method, the first derivative spectra were calculated for ratio spectra generated by dividing the absorption spectra of ternary mixtures containing increasing concentrations of one of the components by a standard spectrum of binary mixture of the other two components (double divisor). The calibration graphs were constructed by measuring the amplitude at either the minimum or maximum wavelengths against the concentrations. The selected wavelengths for determination of EMPA and MET are 282.92 and 250 nm, respectively. The second problem arises from the presence of LINA and EMPA as minor components in their recently approved dosage form with MET thus, sample enrichment through spiking was employed for LINA and EMPA to enable their spectrophotometric analysis in laboratory prepared mixtures that have the same ratio of the three components as in their pharmaceutical dosage form.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: May 4, 2022

Keywords: Linagliptin (LINA); Empaglifozin (EMPA); Metformin HCl (MET); spectrophotometric; Enrichment

References

  • Effect of linagliptin vs placebo on major cardiovascular events in adults with type 2 diabetes and high cardiovascular and renal risk: the CARMELINA randomized clinical trial
  • Efficacy and safety of Linagliptin (tradjenta) in adults with type-2 diabetes mellitus
  • An update on sodium-glucose co-transporter-2 inhibitors for the treatment of diabetes mellitus
  • Cardiovascular and renal outcomes with empagliflozin in heart failure
  • The mechanisms of action of metformin
  • Validated Liquid Chromatographic Method for the Determination of (Canagliflozin, Dapagliflozin or Empagliflozin) and Metformin in the Presence of (1-Cyanoguanidine)
  • A new validated RP-HPLC method for the determination of Metformin HCL and Empagliflozin in its bulk and pharmaceutical dosage form
  • Spectrophotometric methods for the determination of Linagliptin in binary mixture with Metformin Hydrochloride and simultaneous determination of Linagliptin and Metformin Hydrochloride using high performance liquid chromatography
  • Simultaneous determination of Linagliptin and Metformin by reversed phase-high performance liquid chromatography method: An application in quantitative analysis of pharmaceutical dosage forms
  • Determination of novel promising combination of Linagliptin and Pioglitazone HCl in bulk and laboratory synthetic mixture by earth-friendly three spectrophotometric methods
  • Development and validation of UV spectrophotometric method for simultaneous estimation of Empagliflozin and Metformin hydrochloride in bulk drugs and combined dosage forms
  • Development and validation of simple spectrophotometric and chemometric methods for simultaneous determination of Empagliflozin and Metformin: Applied to recently approved pharmaceutical formulation
  • Development of economic UV spectrophotometric method for determination of linagliptin in its ternary mixture with empagliflozin and metformin: Comparison to economic pharmaceutical analysis in literature
  • A new cost effective first derivative spectrophotometric method for assay of linagliptin in combination with selected antidiabetics: Compared to previously reported cost effective procedures
  • Mean centering-triple divisor and ratio derivative-zero crossing for simultaneous determination of some diabetes drugs in their quaternary mixture with severely overlapping spectra
  • UPLC simultaneous determination of empagliflozin, linagliptin and metformin
  • Simultaneous quantifcation of Empaglifozin, Linagliptin, and Metformin hydrochloride in bulk and synthetic mixture by RP-LC method
  • The spectrophotometric multicomponent analysis of a ternary mixture of paracetamol, aspirin, and caffeine by the double divisor-ratio spectra derivative method
  • Different ratio spectra methods for simultaneous determination of Olmesartan Medoxomil, Hydrochlorothiazide and Amlodipine Besylate
  • Sample enrichment of Canagliflozin prior to its spectrophotometric determination in presence of Metformin: Application to recently approved binary dosage form
  • A comparative study of the novel ratio difference method versus conventional spectrophotometric techniques for the analysis of binary mixture with overlapped spectra