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RP-HPLC-PDAAnalyses of Tapentadol: Application of Experimental Design

RP-HPLC-PDAAnalyses of Tapentadol: Application of Experimental Design AbstractA simple, rapid and sensitive RP-HPLC-PDA method was developed and validated as per ICH guidelines for the analysis of tapentadol in bulk as well as in tablet formulation. The RP-HPLC isocratic method was developed using a column LC-GC Qualisil BDS C-18 (250 mm × 4.6 mm × 5 μm) and methanol - potassium dihydrogen phosphate buffer (pH 7.0) mixture (80:20, v/v) as a mobile phase. The flow rate was retained at 1 ml min-1 and the response was detected at 274 nm. Robustness of the developed method was evaluated using two experimental designs (central composite design and response surface methodology). The effect of factors such as mobile phase composition, flow rate and pH of mobile phase were studied. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

RP-HPLC-PDAAnalyses of Tapentadol: Application of Experimental Design

RP-HPLC-PDAAnalyses of Tapentadol: Application of Experimental Design

Abstract

AbstractA simple, rapid and sensitive RP-HPLC-PDA method was developed and validated as per ICH guidelines for the analysis of tapentadol in bulk as well as in tablet formulation. The RP-HPLC isocratic method was developed using a column LC-GC Qualisil BDS C-18 (250 mm × 4.6 mm × 5 μm) and methanol - potassium dihydrogen phosphate buffer (pH 7.0) mixture (80:20, v/v) as a mobile phase. The flow rate was retained at 1 ml min-1 and the response was detected at 274 nm. Robustness...
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Publisher
Taylor & Francis
Copyright
© 2016 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2015.1068705
Publisher site
See Article on Publisher Site

Abstract

AbstractA simple, rapid and sensitive RP-HPLC-PDA method was developed and validated as per ICH guidelines for the analysis of tapentadol in bulk as well as in tablet formulation. The RP-HPLC isocratic method was developed using a column LC-GC Qualisil BDS C-18 (250 mm × 4.6 mm × 5 μm) and methanol - potassium dihydrogen phosphate buffer (pH 7.0) mixture (80:20, v/v) as a mobile phase. The flow rate was retained at 1 ml min-1 and the response was detected at 274 nm. Robustness of the developed method was evaluated using two experimental designs (central composite design and response surface methodology). The effect of factors such as mobile phase composition, flow rate and pH of mobile phase were studied.

Journal

Analytical Chemistry LettersTaylor & Francis

Published: May 3, 2016

Keywords: Tapentadol; RP-HPLC; Central Composite Design; Design of expert; Validation; Photodiode detector

References