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Simultaneous Determination of Acetaminophen and Tramadol Impurities in combination product of Acetaminophen and Tramadol Tablets by UPLC with Trifunctional Octadecyl Column

Simultaneous Determination of Acetaminophen and Tramadol Impurities in combination product of... AbstractA Simple, Precise, Sensitive and time efficient stability indicating reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) method was developed and validated for the simultaneous determination of Acetaminophen and Tramadol impurities present in fixed dose combination product. The components were separated in gradient elution mode using 0.1% Perchloric acid in water and acetonitrile as mobile phase. The column used was Acquity HSS T3 (100 mm x 2.1 mm, 1.8 µ) at a flow rate of 0.5 mL.min−1 with 30°C temperature. Quantification was achieved with detection wavelength of 215 nm. The developed method was validated for specificity, linearity, precision, accuracy, sensitivity (LOD & LOQ) and robustness in accordance with International Conference on Harmonisation (ICH) guidelines. The method is rapid, cost effective for the quantitative determination of Acetaminophen and Tramadol impurities in Acetaminophen and Tramadol tablets dosage forms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Simultaneous Determination of Acetaminophen and Tramadol Impurities in combination product of Acetaminophen and Tramadol Tablets by UPLC with Trifunctional Octadecyl Column

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Simultaneous Determination of Acetaminophen and Tramadol Impurities in combination product of Acetaminophen and Tramadol Tablets by UPLC with Trifunctional Octadecyl Column

Abstract

AbstractA Simple, Precise, Sensitive and time efficient stability indicating reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) method was developed and validated for the simultaneous determination of Acetaminophen and Tramadol impurities present in fixed dose combination product. The components were separated in gradient elution mode using 0.1% Perchloric acid in water and acetonitrile as mobile phase. The column used was Acquity HSS T3 (100 mm x 2.1 mm,...
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Publisher
Taylor & Francis
Copyright
© 2015 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2015.1128849
Publisher site
See Article on Publisher Site

Abstract

AbstractA Simple, Precise, Sensitive and time efficient stability indicating reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) method was developed and validated for the simultaneous determination of Acetaminophen and Tramadol impurities present in fixed dose combination product. The components were separated in gradient elution mode using 0.1% Perchloric acid in water and acetonitrile as mobile phase. The column used was Acquity HSS T3 (100 mm x 2.1 mm, 1.8 µ) at a flow rate of 0.5 mL.min−1 with 30°C temperature. Quantification was achieved with detection wavelength of 215 nm. The developed method was validated for specificity, linearity, precision, accuracy, sensitivity (LOD & LOQ) and robustness in accordance with International Conference on Harmonisation (ICH) guidelines. The method is rapid, cost effective for the quantitative determination of Acetaminophen and Tramadol impurities in Acetaminophen and Tramadol tablets dosage forms.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Sep 3, 2015

Keywords: Stability Indicating; RP-UPLC; Acetaminophen; Tramadol; UV; ICH

References

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