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Simultaneous HPLC Determination of Four α-Blocker Drugs Using Time Programming Technique

Simultaneous HPLC Determination of Four α-Blocker Drugs Using Time Programming Technique AbstractA sensitive and simple reversed-phase high-performance liquid chromatographic method (RP-HPLC) was developed and validated for the simultaneous determination of four α-adrenergic receptor blockers: terazosin (TER), alfuzosin (ALF), tamsulosin (TAM) and doxazosin (DOX) using moxifloxacin (MOX) as an internal standard. The chromatographic separation was carried out isocratically at 40°C on a Prontosil C-18 reversed-phase column with a mobile phase consisting of acetonitrile : 50 mM phosphate buffer in a ratio of 30:70 with pH 3 adjusted by o-phosphoric acid. The analysis was performed in less than 8 min at a flow rate of 1.0 mL/min for terazosin and alfuzosin, and a flow rate of 1.5 mL/min for tamsulosin and doxazosin using time programming technique. UV-detection was performed at 230 nm. The proposed method was linear over concentration ranges of 2.5 - 25.0 μg/mL for TER, ALF, TAM and DOX, with limits of detection of 0.23, 0.17, 0.20 and 0.28 μg/mL in a respective order. The developed method was successfully applied to determine the four drugs in their dosage forms and the results were satisfactory as revealed by statistical analysis of the data. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Simultaneous HPLC Determination of Four α-Blocker Drugs Using Time Programming Technique

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Simultaneous HPLC Determination of Four α-Blocker Drugs Using Time Programming Technique

Abstract

AbstractA sensitive and simple reversed-phase high-performance liquid chromatographic method (RP-HPLC) was developed and validated for the simultaneous determination of four α-adrenergic receptor blockers: terazosin (TER), alfuzosin (ALF), tamsulosin (TAM) and doxazosin (DOX) using moxifloxacin (MOX) as an internal standard. The chromatographic separation was carried out isocratically at 40°C on a Prontosil C-18 reversed-phase column with a mobile phase consisting of acetonitrile :...
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Publisher
Taylor & Francis
Copyright
© 2017 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2017.1372210
Publisher site
See Article on Publisher Site

Abstract

AbstractA sensitive and simple reversed-phase high-performance liquid chromatographic method (RP-HPLC) was developed and validated for the simultaneous determination of four α-adrenergic receptor blockers: terazosin (TER), alfuzosin (ALF), tamsulosin (TAM) and doxazosin (DOX) using moxifloxacin (MOX) as an internal standard. The chromatographic separation was carried out isocratically at 40°C on a Prontosil C-18 reversed-phase column with a mobile phase consisting of acetonitrile : 50 mM phosphate buffer in a ratio of 30:70 with pH 3 adjusted by o-phosphoric acid. The analysis was performed in less than 8 min at a flow rate of 1.0 mL/min for terazosin and alfuzosin, and a flow rate of 1.5 mL/min for tamsulosin and doxazosin using time programming technique. UV-detection was performed at 230 nm. The proposed method was linear over concentration ranges of 2.5 - 25.0 μg/mL for TER, ALF, TAM and DOX, with limits of detection of 0.23, 0.17, 0.20 and 0.28 μg/mL in a respective order. The developed method was successfully applied to determine the four drugs in their dosage forms and the results were satisfactory as revealed by statistical analysis of the data.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Jul 4, 2017

Keywords: Alpha blockers; RP-HPLC; time programming; pharmaceutical preparation

References

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