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Spectroscopic and Molecular Docking Investigations on the Effect of the Interaction Between Cefoperazone Sodium and Papain to Drug Efficacy

Spectroscopic and Molecular Docking Investigations on the Effect of the Interaction Between... AbstractUnder the experimental condition of pH=7.40, papain (PAPA) was used as the detection object, and the binding behavior of cefoperazone sodium (CFZ) and PAPA at three temperatures was studied by multi-spectroscopy and molecular docking. The results showed that CFZ can effectively quench the endogenous fluorescence of PAPA, form a 1:1 complex, and change the conformation of PAPA. At 293 K, the fluorescence intensity of the system had a good linear relationship with the CFZ concentration in the range of 4.0×10-6 ∼ 1.0×10-4 mol/L. The detection limit of the method was 2.43×10-6 mol/L (n=10), indicating that the reaction can be used to achieve rapid determination of CFZ content in the actual drug. Based on the experimental data, a mathematical model combining CFZ and PAPA was established. When the peak plasma concentration of CFZ was 106.0 mg/L after intravenous infusion of 1 g, the drug binding rate of CFZ-PAPA system was 2.70 %, indicating the combination of CFZ and PAPA did not affect the efficacy of CFZ, that is, when taking CFZ, it is safe to consume PAPA. Molecular simulation studies had showed that the interaction between CFZ and PAPA had not only electrostatic forces but also hydrogen bonding, which was consistent with the results of spectroscopy and thermodynamic studies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Spectroscopic and Molecular Docking Investigations on the Effect of the Interaction Between Cefoperazone Sodium and Papain to Drug Efficacy

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Spectroscopic and Molecular Docking Investigations on the Effect of the Interaction Between Cefoperazone Sodium and Papain to Drug Efficacy

Abstract

AbstractUnder the experimental condition of pH=7.40, papain (PAPA) was used as the detection object, and the binding behavior of cefoperazone sodium (CFZ) and PAPA at three temperatures was studied by multi-spectroscopy and molecular docking. The results showed that CFZ can effectively quench the endogenous fluorescence of PAPA, form a 1:1 complex, and change the conformation of PAPA. At 293 K, the fluorescence intensity of the system had a good linear relationship with the CFZ concentration...
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Publisher
Taylor & Francis
Copyright
© 2019 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2019.1605929
Publisher site
See Article on Publisher Site

Abstract

AbstractUnder the experimental condition of pH=7.40, papain (PAPA) was used as the detection object, and the binding behavior of cefoperazone sodium (CFZ) and PAPA at three temperatures was studied by multi-spectroscopy and molecular docking. The results showed that CFZ can effectively quench the endogenous fluorescence of PAPA, form a 1:1 complex, and change the conformation of PAPA. At 293 K, the fluorescence intensity of the system had a good linear relationship with the CFZ concentration in the range of 4.0×10-6 ∼ 1.0×10-4 mol/L. The detection limit of the method was 2.43×10-6 mol/L (n=10), indicating that the reaction can be used to achieve rapid determination of CFZ content in the actual drug. Based on the experimental data, a mathematical model combining CFZ and PAPA was established. When the peak plasma concentration of CFZ was 106.0 mg/L after intravenous infusion of 1 g, the drug binding rate of CFZ-PAPA system was 2.70 %, indicating the combination of CFZ and PAPA did not affect the efficacy of CFZ, that is, when taking CFZ, it is safe to consume PAPA. Molecular simulation studies had showed that the interaction between CFZ and PAPA had not only electrostatic forces but also hydrogen bonding, which was consistent with the results of spectroscopy and thermodynamic studies.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Mar 4, 2019

Keywords: Cfzoperazone sodium; papain; spectroscopic methods; molecular docking

References

  • Determination of the kinetics and influence of the mercury ion on papain catalytic activity
  • Design and development of papain- urea loaded PVA nanofibers for wound debridement
  • Chemical modification of papain for use in alkaline medium J
  • Multicenter clinical trial of cefopera- zone sodium in the United States
  • Solubility and Metastable Zone of Cfzopera- zone Sodium in Acetone plus Water system
  • Spectroscopic study on the interaction of Trypsin with Bicyclol and analogs
  • Study on the competitive reaction between bovine serum albumin and neomycin with ponceau S as fluorescence probe
  • Studies on the binding of fulvic acid with transferrin by spectroscopic analysis J
  • Comparative studies on the interaction of cefixime with bovine serum albumin by fluorescence quenching spectroscopy and synchronous fluorescence spectroscopy
  • Binding of an Anticancer Drug, Axitinib to Human Serum: Fluorescence Quenching and Molecular Docking Study
  • Interaction of cinnamic acid derivatives with serum albumins: a fluorescence spectroscopic study
  • Spectroscopic studies on the interactions between imidazolium chloride ionic liquids and bovine serum albumin
  • Synthesis and spectroscopic characterization of 4-butoxyethoxy-N-octadecyl-1, 8- naphthalimide as a new fluorescent probe for the determination of proteins J
  • Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale
  • Study on the interaction between isoniazid and bovine serum albumin by fluorescence spectroscopy: the effect of dimethylsulfoxide
  • Characteri- zation of the binding and conformational changes of bovine serum albumin upon interaction with antihypertensive olmesartan medoxomil
  • Investigation on the Interaction Between PAPAzyme and cfzepime Hydrochloride by synchronous Fluorescence and Fluorescence Quenching Spectroscopy
  • Study on the interaction between anti tuberculosis drug ethambutol and bovine serum albumin: multispectroscopic and cyclic voltammetric approaches
  • Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine
  • Evaluation of the binding interaction between bovine serum albumin and dimethyl fumarate, an anti-inflammatory drug by multispectroscopic methods
  • A spectroscopic and thermal stability study on the interaction between putrescine and bovine trypsin
  • Study on the conjugation mechanism of colistin sulfate with bovine serum albumin and effect of the metal ions on the reaction
  • Study of interaction of silver nanoparticles with bovine serum albumin using fluorescence spectroscopy
  • The influence of dietary habits and pathological conditions on the binding of theophylline to serum albumin
  • Spectroscopic Studies on the Interaction of CFZ nthetic Food Colorants with Bovine Serum Albumin
  • A forecast of the binding rate of drug to plasma protein by fluorescence quenching method
  • Study of microheterogeneous environ- ment of protein human serum albumin by an extrinsic fluorescent reporter: a spectroscopic study in combination with molecular docking and molecular dynamics simulation