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Spectroscopic Study on the Interaction of Haloperidol and 2,4-Dinitrophenylhydrazine and its Application for the Quantification in Drug Formulations

Spectroscopic Study on the Interaction of Haloperidol and 2,4-Dinitrophenylhydrazine and its... AbstractA simple and sensitive spectrophotometric procedure has been developed for the determination of haloperidol in bulk and its formulation/tablets. Method is based on the interaction of carbonyl group of haloperidol with 2,4-dinitrophenyl hydrazine [DNPH]. The condensation reaction produces a red coloured product which absorbs maximally at 456 nm. The effects of variables such as concentration of DNPH, KOH, HCl and the reaction time were investigated to optimize the procedure. Beer’s law was obeyed in the concentration range of 3.6–10.8 μg mL-1 with molar absorptivity of 2.84×104 L mol-1 cm-1. The results were validated statistically as per the ICH Guidelines. The proposed method has been applied to the determination of haloperidol in tablets with satisfactory results. The obtained results are compared statistically with those given by a reference method. The point and interval hypothesis results indicate that the proposed procedure is not biased. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Chemistry Letters Taylor & Francis

Spectroscopic Study on the Interaction of Haloperidol and 2,4-Dinitrophenylhydrazine and its Application for the Quantification in Drug Formulations

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Spectroscopic Study on the Interaction of Haloperidol and 2,4-Dinitrophenylhydrazine and its Application for the Quantification in Drug Formulations

Rahman, Nafisur; Sameen, Shahroora; Kashif, Mohammad
Analytical Chemistry Letters , Volume 6 (6): 12 – Nov 1, 2016

Abstract

AbstractA simple and sensitive spectrophotometric procedure has been developed for the determination of haloperidol in bulk and its formulation/tablets. Method is based on the interaction of carbonyl group of haloperidol with 2,4-dinitrophenyl hydrazine [DNPH]. The condensation reaction produces a red coloured product which absorbs maximally at 456 nm. The effects of variables such as concentration of DNPH, KOH, HCl and the reaction time were investigated to optimize the procedure. Beer’s law was obeyed in the concentration range of 3.6–10.8 μg mL-1 with molar absorptivity of 2.84×104 L mol-1 cm-1. The results were validated statistically as per the ICH Guidelines. The proposed method has been applied to the determination of haloperidol in tablets with satisfactory results. The obtained results are compared statistically with those given by a reference method. The point and interval hypothesis results indicate that the proposed procedure is not biased.

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Publisher
Taylor & Francis
Copyright
© 2016 Har Krishan Bhalla & Sons
ISSN
2230-7532
eISSN
2229-7928
DOI
10.1080/22297928.2016.1265898
Publisher site
See Article on Publisher Site

Abstract

AbstractA simple and sensitive spectrophotometric procedure has been developed for the determination of haloperidol in bulk and its formulation/tablets. Method is based on the interaction of carbonyl group of haloperidol with 2,4-dinitrophenyl hydrazine [DNPH]. The condensation reaction produces a red coloured product which absorbs maximally at 456 nm. The effects of variables such as concentration of DNPH, KOH, HCl and the reaction time were investigated to optimize the procedure. Beer’s law was obeyed in the concentration range of 3.6–10.8 μg mL-1 with molar absorptivity of 2.84×104 L mol-1 cm-1. The results were validated statistically as per the ICH Guidelines. The proposed method has been applied to the determination of haloperidol in tablets with satisfactory results. The obtained results are compared statistically with those given by a reference method. The point and interval hypothesis results indicate that the proposed procedure is not biased.

Journal

Analytical Chemistry Letters – Taylor & Francis

Published: Nov 1, 2016

Keywords: Haloperidol; Spectrophotometry; 2,4-Dinitrophenyl hydrazine; Pharmaceutical Formulation

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