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The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water fish aquarium

The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water... An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell, cell division in non-motile cysts, and a theca composed of thin but structured plates. Morphological analysis of flagellate cells by scanning electron microscopy revealed a Kofoid thecal plate tabulation of 4', 4a, 4", 'X', 5 or 6c, ?s, 5"', 1p, 1"", most consistent with the original description of Crypthecodinium setense Biecheler. This Crypthecodinium species exhibited a high maximum division rate (3.2 divisions day−1) and cell yield (>106 cells ml−1) when fed cultured fish cells. Small sub-unit rDNA phylogenetic analyses supported relatedness with a previously studied Crypthecodinium-like dinoflagellate, but a significant difference in aligned gene sequences was found. This study provides the first clear demonstration of the plate tabulation of a Crypthecodinium species since the original description over 60 years ago, allowing the original morphological conception of Crypthecodinium to be linked with molecular phylogenetic information. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png African Journal of Marine Science Taylor & Francis

The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water fish aquarium

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The taxonomy and growth of a Crypthecodinium species (Dinophyceae) isolated from a brackish-water fish aquarium

Abstract

An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell, cell division in non-motile cysts, and a theca composed of thin but structured plates. Morphological analysis of flagellate cells by scanning electron microscopy revealed a Kofoid thecal plate...
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Publisher
Taylor & Francis
Copyright
Copyright Taylor & Francis Group, LLC
ISSN
1814-2338
eISSN
1814-232X
DOI
10.2989/18142320609504145
Publisher site
See Article on Publisher Site

Abstract

An unidentified heterotrophic dinoflagellate found growing in abundance in a brackish-water fish aquarium was isolated and serially cultivated using a fish cell line as the food source. Prominent characteristics of this dinoflagellate included a cingulum that did not fully encircle the motile cell, cell division in non-motile cysts, and a theca composed of thin but structured plates. Morphological analysis of flagellate cells by scanning electron microscopy revealed a Kofoid thecal plate tabulation of 4', 4a, 4", 'X', 5 or 6c, ?s, 5"', 1p, 1"", most consistent with the original description of Crypthecodinium setense Biecheler. This Crypthecodinium species exhibited a high maximum division rate (3.2 divisions day−1) and cell yield (>106 cells ml−1) when fed cultured fish cells. Small sub-unit rDNA phylogenetic analyses supported relatedness with a previously studied Crypthecodinium-like dinoflagellate, but a significant difference in aligned gene sequences was found. This study provides the first clear demonstration of the plate tabulation of a Crypthecodinium species since the original description over 60 years ago, allowing the original morphological conception of Crypthecodinium to be linked with molecular phylogenetic information.

Journal

African Journal of Marine Science – Taylor & Francis

Published: Sep 1, 2006

Keywords: CRYPTHECODINIUM; DINOFLAGELLATE; PHYLOGENY; SMALL SUB-UNIT RDNA; TAXONOMY; THECA

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