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Urease inhibitory constituents from Daphne retusa

Urease inhibitory constituents from Daphne retusa The bioassay-guided fractionation of Daphne retusa Hemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by 1H NMR, 13C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1–7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound 2 exhibited significant urease inhibition with IC50 value 60.4 ± 0.72 μM, whereas compounds 1 and 3–7 remained inactive during urease inhibition and α-chymotrypsin bioassays. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Asian Natural Products Research Taylor & Francis

Urease inhibitory constituents from Daphne retusa

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References (30)

Publisher
Taylor & Francis
Copyright
© 2013 Taylor & Francis
ISSN
1477-2213
eISSN
1028-6020
DOI
10.1080/10286020.2013.837457
pmid
24266421
Publisher site
See Article on Publisher Site

Abstract

The bioassay-guided fractionation of Daphne retusa Hemsl. has led to the isolation of a new aryl tetrahydronaphthalene lignan derivative named as daphnretusic acid (1), along with six new source compounds such as 5,7-dihydroxyflavone (2), 7-hydroxyflavone (3), 6-methoxyflavone (4), (+) pinoresinol (5), (+) sesamin (6), and β-sitosterol-3-O-β-D-glucopyranoside (7). Their structures were elucidated by 1H NMR, 13C NMR, 1D, 2D NMR, UV, IR, and EIMS analyses. All the fractions (n-hexane, CHCl3, AcOEt, CH3OH, and water) and pure compounds (1–7) were subjected to the assay of urease and α-chymotrypsin inhibitory activities. Chloroform and methanol soluble fractions showed moderate urease inhibition. Compound 2 exhibited significant urease inhibition with IC50 value 60.4 ± 0.72 μM, whereas compounds 1 and 3–7 remained inactive during urease inhibition and α-chymotrypsin bioassays.

Journal

Journal of Asian Natural Products ResearchTaylor & Francis

Published: Feb 1, 2014

Keywords: Daphne retusa; Thymeleaceae; flavones; urease inhibitors; α-chymotrypsin enzyme

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