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Effects of post-training hippocampal injections of midazolam on fear conditioning

Effects of post-training hippocampal injections of midazolam on fear conditioning Downloaded from learnmem.cshlp.org on November 15, 2021 - Published by Cold Spring Harbor Laboratory Press Research Effects of post-training hippocampal injections of midazolam on fear conditioning Georgette M. Gafford, Ryan G. Parsons, and Fred J. Helmstetter Department of Psychology, University of Wisconsin–Milwaukee, Milwaukee, Wisconsin 53201, USA Benzodiazepines have been useful tools for investigating mechanisms underlying learning and memory. The present set of experiments investigates the role of hippocampal GABA /benzodiazepine receptors in memory consolidation using Pavlovian fear conditioning. Rats were prepared with cannulae aimed at the dorsal hippocampus and trained with a series of white noise–shock pairings. In the first experiment, animals received intrahippocampal infusion of midazolam or vehicle immediately or 3 h after training. Then, 24 h later, freezing to the training context and the white noise were measured independently. Results show infusion of midazolam immediately, but not 3 h, after training selectively attenuates contextual fear conditioning. In the second experiment, animals received intrahippocampal infusions of an antisense oligodeoxynucleotide (ODN) targeting the 5 subunit of the GABA receptor or a missense control for several days prior to training and testing. Immediately after training, animals received an infusion of either midazolam or vehicle. Western blots conducted after testing showed http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Learning & Memory Unpaywall

Effects of post-training hippocampal injections of midazolam on fear conditioning

Learning & MemoryNov 1, 2005
7 pages

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Publisher
Unpaywall
ISSN
1072-0502
DOI
10.1101/lm.51305
Publisher site
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Abstract

Downloaded from learnmem.cshlp.org on November 15, 2021 - Published by Cold Spring Harbor Laboratory Press Research Effects of post-training hippocampal injections of midazolam on fear conditioning Georgette M. Gafford, Ryan G. Parsons, and Fred J. Helmstetter Department of Psychology, University of Wisconsin–Milwaukee, Milwaukee, Wisconsin 53201, USA Benzodiazepines have been useful tools for investigating mechanisms underlying learning and memory. The present set of experiments investigates the role of hippocampal GABA /benzodiazepine receptors in memory consolidation using Pavlovian fear conditioning. Rats were prepared with cannulae aimed at the dorsal hippocampus and trained with a series of white noise–shock pairings. In the first experiment, animals received intrahippocampal infusion of midazolam or vehicle immediately or 3 h after training. Then, 24 h later, freezing to the training context and the white noise were measured independently. Results show infusion of midazolam immediately, but not 3 h, after training selectively attenuates contextual fear conditioning. In the second experiment, animals received intrahippocampal infusions of an antisense oligodeoxynucleotide (ODN) targeting the 5 subunit of the GABA receptor or a missense control for several days prior to training and testing. Immediately after training, animals received an infusion of either midazolam or vehicle. Western blots conducted after testing showed

Journal

Learning & MemoryUnpaywall

Published: Nov 1, 2005

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