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MOLECULAR IDENTIFICATION OF Tinospora sinensis BY ITS2 SEQUENCE ANALYSIS

MOLECULAR IDENTIFICATION OF Tinospora sinensis BY ITS2 SEQUENCE ANALYSIS International Journal of Molecular Biology ISSN: 0976-0482 & E-ISSN: 0976-0490, Volume 3, Issue 2, 2012, pp.-55-57. Available online at http://www.bioinfo.in/contents.php?id=34 1 1 1 2 2 1 KHANAPURKAR R.S. , PAUL NILESH ., DESAI D.M. , LINGOJWAR D.P. , RAUT M.R. AND GANGAWANE A.K. Department of Biotechnology, School of Biomedical Science, MGM Institute of Health Sciences, Kamothe, Navi Mumbai, MS, India. ATG Lab, Pune, MS, India. *Corresponding Author: Email- ajganga@yahoo.com Received: April 05, 2012; Accepted: May 21, 2012 Abstract- Intergenic spacer region 2 (ITS2) between 5.8S RNA and 28S RNA is well established genomic region for molecular identification of plant as potential universal barcode. The Present studies deals with molecular identification of Tinospora sinensis, by a unique ITS2 re- gion. Briefly, the plant genomic DNA isolated by CTAB method and targeted for amplification of ITS2 region by eukaryotic conserved primers i.e. RM5.8s1F and RM 28s R for generation of 400 bp amplicon followed by DNA sequencing. Sequence analysis by BLAST search on NCBI database and phylogenetic studies suggests 96% identity with published sequence of Tinospora sinensis, i.e. intraspecies identity and 92% identity with other species of Tinospora genera i.e. T. malabarica. Phylogenetic analysis by MEGA4.1 suggest more close relationship with http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Molecular Biology Unpaywall

MOLECULAR IDENTIFICATION OF Tinospora sinensis BY ITS2 SEQUENCE ANALYSIS

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Publisher
Unpaywall
ISSN
0976-0482
DOI
10.9735/0976-0482.3.2.55-57
Publisher site
See Article on Publisher Site

Abstract

International Journal of Molecular Biology ISSN: 0976-0482 & E-ISSN: 0976-0490, Volume 3, Issue 2, 2012, pp.-55-57. Available online at http://www.bioinfo.in/contents.php?id=34 1 1 1 2 2 1 KHANAPURKAR R.S. , PAUL NILESH ., DESAI D.M. , LINGOJWAR D.P. , RAUT M.R. AND GANGAWANE A.K. Department of Biotechnology, School of Biomedical Science, MGM Institute of Health Sciences, Kamothe, Navi Mumbai, MS, India. ATG Lab, Pune, MS, India. *Corresponding Author: Email- ajganga@yahoo.com Received: April 05, 2012; Accepted: May 21, 2012 Abstract- Intergenic spacer region 2 (ITS2) between 5.8S RNA and 28S RNA is well established genomic region for molecular identification of plant as potential universal barcode. The Present studies deals with molecular identification of Tinospora sinensis, by a unique ITS2 re- gion. Briefly, the plant genomic DNA isolated by CTAB method and targeted for amplification of ITS2 region by eukaryotic conserved primers i.e. RM5.8s1F and RM 28s R for generation of 400 bp amplicon followed by DNA sequencing. Sequence analysis by BLAST search on NCBI database and phylogenetic studies suggests 96% identity with published sequence of Tinospora sinensis, i.e. intraspecies identity and 92% identity with other species of Tinospora genera i.e. T. malabarica. Phylogenetic analysis by MEGA4.1 suggest more close relationship with

Journal

International Journal of Molecular BiologyUnpaywall

Published: May 30, 2012

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