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Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase

Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial... Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars. However, the presence of lignin hinders utilization of these carbohydrates and hence pretreatment to remove lignin is necessarily carried out. Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material. Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL−1 of laccase was obtained. Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL−1. Five factors (incubation period, concentration of CuSO4, temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL−1). Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL−1 of cellulase. Delignification and cellulose utilization were affirmed by structural analysis through FTIR and SEM. The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Basic Microbiology Wiley

Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase

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Publisher
Wiley
Copyright
© 2023 Wiley‐VCH GmbH.
ISSN
0233-111X
eISSN
1521-4028
DOI
10.1002/jobm.202200684
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Abstract

Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars. However, the presence of lignin hinders utilization of these carbohydrates and hence pretreatment to remove lignin is necessarily carried out. Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material. Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL−1 of laccase was obtained. Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL−1. Five factors (incubation period, concentration of CuSO4, temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL−1). Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL−1 of cellulase. Delignification and cellulose utilization were affirmed by structural analysis through FTIR and SEM. The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.

Journal

Journal of Basic Microbiology – Wiley

Published: Mar 1, 2023

Keywords: endoglucanase; laccase; Neobacillus sedimentimangrovi; Trametes pubescens

References

  • Engineering microbial surfaces to degrade lignocellulosic biomass
    Huang, GL; Anderson, TD; Clubb, RT
  • Production and characterisation of xylanase and endoglucanases produced by Penicillium roqueforti ATCC 10110 through the solid‐state fermentation of rice husk residue
    Marques, GL; Santos Reis, N; Silva, TP; Ferreira, MLO; Aguiar‐Oliveira, E; Oliveira, JR
  • Bioconversion of sugarcane biomass into ethanol: an overview about composition, pretreatment methods, detoxification of hydrolysates, enzymatic saccharification, and ethanol fermentation
    Canilha, L; Chandel, AK; Santos Milessi, TS; Antunes, FAF; Costa Freitas, WL; Gracas Almeida Felipe, M
  • Cogeneration through bagasse: a renewable strategy to meet the future energy needs
    Arshad, M; Ahmed, S
  • Characterization of cellulases from thermophilic bacilli and their application for the saccharification of sugarcane bagasse
    Hassan, M; Sohail, M
  • Supporting role of lignin in immobilization of yeast on sugarcane bagasse for continuous pectinase production
    Ejaz, U; Sohail, M
  • Comparative study of alkali‐ and acidic organic solvent‐soluble hemicellulosic polysaccharides from sugarcane bagasse
    Xu, F; Sun, JX; Liu, CF; Sun, RC
  • Combined pretreatment of sugarcane bagasse using alkali and ionic liquid to increase hemicellulose content and xylanase production
    Rashid, R; Ejaz, U; Ali, FI; Hashmi, IA; Bari, A; Liu, J
  • Statistical optimization of saccharificaion of carbohydrate content of alkali pretreated sugarcane bagasse by enzyme cocktail produced by Bacillus vallismortis MH 1 and Bacillus aestuarii UE25
    Ejaz, U; Shazad, Y; Hassan, M; Sohail, M
  • Ionic liquid pretreatment as emerging approaches for enhanced enzymatic hydrolysis of lignocellulosic biomass
    Elgharbawy, AA; Alam, MZ; Moniruzzaman, M; Goto, M
  • Production of multienzyme by Bacillus aestuarii UE25 using ionic liquid pretreated sugarcane bagasse
    Zafar, H; Rehman, I; Ejaz, U; Ansari, A; Sohail, M
  • Biological pretreatment of lignocellulosic biomass: an overview
    Sindhu, R; Binod, P; Pandey, A
  • Fungal laccases: production, function, and applications in food processing
    Brijwani, K; Rigdon, A; Vadlani, PV
  • Fungal laccases: the forefront of enzymes for sustainability
    Loi, M; Glazunova, O; Fedorova, T; Logrieco, AF; Mulè, G
  • Application of carbon adsorbents prepared from Brazilian‐pine fruit shell for the removal of reactive orange 16 from aqueous solution: kinetic, equilibrium, and thermodynamic studies
    Calvete, T; Lima, EC; Cardoso, NF; Vaghetti, JCP; Dias, SLP; Pavan, FA
  • Biological bleaching of chemical pulps
    Bajpai, P
  • Enhanced formation of laccase activity by the white‐rot fungus Trametes pubescens in the presence of copper
    Galhaup, C; Haltrich, D
  • Coupling laccase production from trametes pubescence with heavy metal removal for economic waste water treatment
    Enayatizamir, N; Liu, J; Wang, L; Lin, X; Fu, P
  • Laccase‐mediated delignification and detoxification of lignocellulosic biomass: removing obstacles in energy generation
    Malhotra, M; Suman, SK
  • Optimization of inulinase production by solid‐state fermentation using sugarcane bagasse as substrate
    Mazutti, M; Bender, JP; Treichel, H; Luccio, MD
  • Thermostable enzymes in lignocellulose hydrolysis
    Viikari, L; Alapuranen, M; Puranen, T; Vehmaanperä, J; Siika‐Aho, M
  • Methyltrioctylammonium chloride mediated removal of lignin from sugarcane bagasse for themostable cellulase production
    Ejaz, U; Muhammad, S; Ali, FI; Hashmi, IA; Sohail, M
  • Use of dinitrosalicylic acid reagent for determination of reducing sugar
    Miller, GL
  • Comparison of the biochemical properties between the xylanases of Thermomyces lanuginosus (Sigma®) and excreted by Penicillium roqueforti ATCC 10110 during the solid state fermentation of sugarcane bagasse
    Souza, LO; Brito, AR; Bonomo, RCF; Santana, NB; Almeida Antunes Ferraz, JL; Aguiar‐Oliveira, E
  • Use of ionic liquid pretreated and fermented sugarcane bagasse as an adsorbent for congo red removal
    Ejaz, U; Wasim, AA; Khan, MN; Alzahrani, OM; Mahmoud, SF; El‐Bahy, ZM
  • Production, optimisation and partial characterisation of enzymes from filamentous fungi using dried forage cactus pear as substrate
    Santos, TC; Santos Reis, N; Silva, TP; Bonomo, RCF; Aguiar‐Oliveira, E; Oliveira, JR
  • Enzymes for second generation biofuels: recent developments and future perspectives
    Binod, P; Gnansounou, E; Sindhu, R; Pandey, A
  • Harnessing the biocatalytic attributes and applied perspectives of nanoengineered laccases—a review
    Bilal, M; Ashraf, SS; Cui, J; Lou, WY; Franco, M; Mulla, SI
  • Comparative study on the determination of assay for laccase of Trametes sp
    Li, A; Zhu, Y; Xu, L; Zhu, W; Tian, X
  • Development of co‐immobilized tri‐enzyme biocatalytic system for one‐pot pretreatment of four different perennial lignocellulosic biomass and evaluation of their bioethanol production potential
    Kirupa Sankar, M; Ravikumar, R; Naresh Kumar, M; Sivakumar, U
  • Enzymatic delignification of sugar cane bagasse and rice husks and its effect in saccharification
    Matei, JC; Soares, M; Bonato, ACH; Freitas, MPA; Helm, CV; Maroldi, WV
  • Increased production of laccase by the wood‐degrading basidiomycete Trametes pubescens
    Galhaup, C; Wagner, H; Hinterstoisser, B; Haltrich, D
  • Production of Trametes pubescens laccase under submerged and semi‐solid culture conditions on agro‐industrial wastes
    Gonzalez, JC; Medina, SC; Rodriguez, A; Osma, JF; Alméciga‐Díaz, CJ; Sánchez, OF
  • Lignocellulosic residues: biodegradation and bioconversion by fungi
    Sánchez, C
  • Production and extraction optimization of xylanase from Aspergillus niger DFR‐5 through solid‐state‐fermentation
    Pal, A; Khanum, F
  • Laccase production by Coriolopsis caperata RCK2011: optimization under solid state fermentation by Taguchi DOE methodology
    Nandal, P; Ravella, SR; Kuhad, RC
  • Identification of a novel copper‐activated and halide‐tolerant laccase in Geobacillus thermopakistaniensis
    Basheer, S; Rashid, N; Ashraf, R; Akram, MS; Siddiqui, MA; Imanaka, T
  • Factors affecting laccase production by Pleurotus ostreatus and Pleurotus sajor‐caju
    Abo‐State, MAM; Khatab, O; Abo‐El Nasar, A; Mahmoud, B
  • The manganese peroxidase gene family of Trametes trogii: gene identification and expression patterns using various metal ions under different culture conditions
    Zhang, Y; Dong, Z; Luo, Y; Yang, E; Xu, H; Chagan, I
  • Studies on the effect of particle size on solid‐state fermentation of sugarcane bagasse into animal feed using white‐rot fungi
    Zadrazil, F; Puniya, AK
  • Production of laccase enzyme for their potential application to decolorize fungal pigments on aging paper and parchment
    Abd El Monssef, RA; Hassan, EA; Ramadan, EM
  • Laccase production from a temperature and pH tolerant fungal strain of Trametes hirsuta (MTCC 11397)
    Dhakar, K; Pandey, A
  • Optimization of laccase production from a novel strain‐Streptomyces psammoticus using response surface methodology
    Niladevi, KN; Sukumaran, RK; Jacob, N; Anisha, GS; Prema, P
  • Biocatalytic versatilities and biotechnological prospects of laccase for a sustainable industry
    Ayodeji, FD; Shava, B; Iqbal, HMN; Ashraf, SS; Cui, J; Fraco, M
  • Extraction and characterization of cellulose from halophytes: next generation source of cellulose fibre
    Singh, A; Ranawat, B; Meena, R