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Comparison of the fluorescent redox dye 5‐cyano‐2,3‐ditolyltetrazolium chloride with p‐iodonitrotetrazolium violet to detect metabolic activity in heat‐stressed Listeria monocytogenes cells

Comparison of the fluorescent redox dye 5‐cyano‐2,3‐ditolyltetrazolium chloride with... The fluorogenic redox indicator 5‐cyano‐2,3‐ditolyltetrazolium chloride (CTC) was compared with the chromogenic p‐iodonitrotetrazolium violet (INT) and conventional methods to assess cellular viability. Mild heat treatment was used as a well‐controlled method for producing non‐viable and sub‐lethally injured cells. CTC gave an underestimation of the viability of Listeria monocytogenes cells when compared with classical plating methods whereas INT gave an overestimation. However, CTC proved to be a sensitive indicator of uninjured cells. The difference between the total count and the CTC count was equivalent to the injured cell population. The fluorescent formazan formed on reduction ofCTC was readily detected with a charge coupled device and cells enumerated automatically using image analysis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Applied Microbiology Wiley

Comparison of the fluorescent redox dye 5‐cyano‐2,3‐ditolyltetrazolium chloride with p‐iodonitrotetrazolium violet to detect metabolic activity in heat‐stressed Listeria monocytogenes cells

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References (21)

Publisher
Wiley
Copyright
"Copyright © 1994 Wiley Subscription Services, Inc., A Wiley Company"
ISSN
1364-5072
eISSN
1365-2672
DOI
10.1111/j.1365-2672.1994.tb03434.x
Publisher site
See Article on Publisher Site

Abstract

The fluorogenic redox indicator 5‐cyano‐2,3‐ditolyltetrazolium chloride (CTC) was compared with the chromogenic p‐iodonitrotetrazolium violet (INT) and conventional methods to assess cellular viability. Mild heat treatment was used as a well‐controlled method for producing non‐viable and sub‐lethally injured cells. CTC gave an underestimation of the viability of Listeria monocytogenes cells when compared with classical plating methods whereas INT gave an overestimation. However, CTC proved to be a sensitive indicator of uninjured cells. The difference between the total count and the CTC count was equivalent to the injured cell population. The fluorescent formazan formed on reduction ofCTC was readily detected with a charge coupled device and cells enumerated automatically using image analysis.

Journal

Journal of Applied MicrobiologyWiley

Published: Oct 1, 1994

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