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Functional genomics of the human epididymis: Further characterization of efferent ducts and model systems by single‐cell RNA sequencing analysis

Functional genomics of the human epididymis: Further characterization of efferent ducts and model... INTRODUCTIONIt is well known that the epididymis of each species has a subtly different anatomy and physiology, though all are adapted to ensure effective sperm maturation and reproductive success.1 Many insights on epididymis function come from rodents and larger mammals such as the pig2–9 which are particularly well studied but may not accurately represent the human organ. Over the past few years, we have made a concerted effort to study the human epididymis using functional genomics protocols in concert with primary cell10–15 and organoid16 models to investigate the biology of the human organ and its response to androgens. There are multiple challenges in researching the human epididymis: first, the paucity of human tissue samples; second, the apparent lack of complete septa dividing the functional regions of the human epididymis making characterization of each region somewhat problematic (septa have been observed,17–19 though their biological properties are not fully characterized); third, the dramatic diversity in structure among different individuals. Extensive morphological and anatomical studies on the human organ have provided a valuable encyclopedia of the human epididymis (reviewed in19,20), however, in real‐time it is often difficult to correlate this information with fresh tissue samples when histology is not possible.The human epididymis http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Andrology Wiley

Functional genomics of the human epididymis: Further characterization of efferent ducts and model systems by single‐cell RNA sequencing analysis

Leir, Shih‐Hsing; Paranjapye, Alekh; Harris, Ann
Andrology , Volume Early View – Jun 10, 2023
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References (30)

Publisher
Wiley
Copyright
© 2023 American Society of Andrology and European Academy of Andrology.
ISSN
2047-2919
eISSN
2047-2927
DOI
10.1111/andr.13477
Publisher site
See Article on Publisher Site

Abstract

INTRODUCTIONIt is well known that the epididymis of each species has a subtly different anatomy and physiology, though all are adapted to ensure effective sperm maturation and reproductive success.1 Many insights on epididymis function come from rodents and larger mammals such as the pig2–9 which are particularly well studied but may not accurately represent the human organ. Over the past few years, we have made a concerted effort to study the human epididymis using functional genomics protocols in concert with primary cell10–15 and organoid16 models to investigate the biology of the human organ and its response to androgens. There are multiple challenges in researching the human epididymis: first, the paucity of human tissue samples; second, the apparent lack of complete septa dividing the functional regions of the human epididymis making characterization of each region somewhat problematic (septa have been observed,17–19 though their biological properties are not fully characterized); third, the dramatic diversity in structure among different individuals. Extensive morphological and anatomical studies on the human organ have provided a valuable encyclopedia of the human epididymis (reviewed in19,20), however, in real‐time it is often difficult to correlate this information with fresh tissue samples when histology is not possible.The human epididymis

Journal

Andrology – Wiley

Published: Jun 10, 2023

Keywords: caput epididymis; cell identity; efferent ducts; human epididymis epithelium; scRNA‐seq

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