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Minimal residual disease monitoring in chronic lymphocytic leukaemia patients. A comparative analysis of flow cytometry and ASO IgH RQ‐PCR

Minimal residual disease monitoring in chronic lymphocytic leukaemia patients. A comparative... Minimal residual disease (MRD) is becoming increasingly important in chronic lymphocytic leukaemia (CLL) as treatment strategies are progressively improving. The primary objective of this study was to compare the applicability of three different flow cytometric approaches: basic 4‐colour analysis, European Research Initiative in CLL (ERIC) consensus method and 8‐colour analysis. Secondly, we investigated the sensitivity and specificity of flow cytometry (FC) compared to molecular analyses for MRD detection. A total of 462 CLL samples were evaluated by basic FC; in 143, ERIC consensus method was also performed and all three FC methodologies were applied in a subgroup of 10 cases. No discordance in defining MRD‐positive/negative samples was observed between the FC methods; within positive samples, the ERIC consensus method and 8‐colour analysis showed the most accurate results. MRD was analysed by FC and polymerase chain reaction (PCR) in 243 cases: concordant results were obtained in 199/243 samples (81·9%); 42/243 were FC−/PCR+. Overall, the sensitivity and specificity of FC compared to PCR was 96·5% and 77·2%, respectively. Both FC and PCR proved suitable for the detection of MRD and prediction of progression‐free survival, which was significantly reduced in MRD‐positive patients, regardless of the methodology. These results offer the rationale for a strategy to monitor MRD in CLL patients. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png British Journal of Haematology Wiley

Minimal residual disease monitoring in chronic lymphocytic leukaemia patients. A comparative analysis of flow cytometry and ASO IgH RQ‐PCR

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References (30)

Publisher
Wiley
Copyright
Copyright © 2014 John Wiley & Sons Ltd
ISSN
0007-1048
eISSN
1365-2141
DOI
10.1111/bjh.12887
pmid
24735016
Publisher site
See Article on Publisher Site

Abstract

Minimal residual disease (MRD) is becoming increasingly important in chronic lymphocytic leukaemia (CLL) as treatment strategies are progressively improving. The primary objective of this study was to compare the applicability of three different flow cytometric approaches: basic 4‐colour analysis, European Research Initiative in CLL (ERIC) consensus method and 8‐colour analysis. Secondly, we investigated the sensitivity and specificity of flow cytometry (FC) compared to molecular analyses for MRD detection. A total of 462 CLL samples were evaluated by basic FC; in 143, ERIC consensus method was also performed and all three FC methodologies were applied in a subgroup of 10 cases. No discordance in defining MRD‐positive/negative samples was observed between the FC methods; within positive samples, the ERIC consensus method and 8‐colour analysis showed the most accurate results. MRD was analysed by FC and polymerase chain reaction (PCR) in 243 cases: concordant results were obtained in 199/243 samples (81·9%); 42/243 were FC−/PCR+. Overall, the sensitivity and specificity of FC compared to PCR was 96·5% and 77·2%, respectively. Both FC and PCR proved suitable for the detection of MRD and prediction of progression‐free survival, which was significantly reduced in MRD‐positive patients, regardless of the methodology. These results offer the rationale for a strategy to monitor MRD in CLL patients.

Journal

British Journal of HaematologyWiley

Published: Jan 1, 2014

Keywords: ; ; ;

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