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Molecular Cloning of PCR Products

Molecular Cloning of PCR Products It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols in Molecular Biology Wiley

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References (13)

Publisher
Wiley
Copyright
© John Wiley and Sons
ISSN
1934-3639
eISSN
1934-3647
DOI
10.1002/0471142727.mb1504s56
Publisher site
See Article on Publisher Site

Abstract

It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high‐quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.

Journal

Current Protocols in Molecular BiologyWiley

Published: Oct 1, 2001

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