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Overview of PCR

Overview of PCR As a means of rapidly copying and amplifying a selected template sequence from a pool of DNA in vitro, the polymerase chain reaction (PCR) as a stand‐alone technique and in combinations with other methods has a vast range of applications. This chapter provides an overview of the theory and applications for this powerful and versatile laboratory method. A generic protocol for the broadest application is described in this unit along with the basic theory underpinning PCR to foster an understanding of how to make modifications to the protocol that can be applied to specific applications of the PCR technique. There is a troubleshooting table provided to describe and resolve the most common problems associated with PCR, as well as a table for suppliers and manufacturers of thermal cycling instruments. A detailed discussion of various DNA polymerases and suppliers is also provided. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols Essential Laboratory Techniques Wiley

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References (73)

Publisher
Wiley
Copyright
© John Wiley and Sons
ISSN
1948-3430
eISSN
1948-3430
DOI
10.1002/9780470089941.et1002s00
Publisher site
See Article on Publisher Site

Abstract

As a means of rapidly copying and amplifying a selected template sequence from a pool of DNA in vitro, the polymerase chain reaction (PCR) as a stand‐alone technique and in combinations with other methods has a vast range of applications. This chapter provides an overview of the theory and applications for this powerful and versatile laboratory method. A generic protocol for the broadest application is described in this unit along with the basic theory underpinning PCR to foster an understanding of how to make modifications to the protocol that can be applied to specific applications of the PCR technique. There is a troubleshooting table provided to describe and resolve the most common problems associated with PCR, as well as a table for suppliers and manufacturers of thermal cycling instruments. A detailed discussion of various DNA polymerases and suppliers is also provided.

Journal

Current Protocols Essential Laboratory TechniquesWiley

Published: Jan 1, 2008

Keywords: polymerase chain reaction (PCR); dNTPs; thermal cycler; DNA polymerase; DNA template; denaturation; annealment; elongation; melting temperature ( Tm ); hot start; primer design; contamination

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