Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British Columbia, Canada

Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British... Pavilion Lake in British Columbia, Canada, is home to modern‐day microbialites that are actively growing at multiple depths within the lake. While microbialite morphology changes with depth and previous isotopic investigations suggested a biological role in the formation of these carbonate structures, little is known about their microbial communities. Microbialite samples acquired through the Pavilion Lake Research Project (PLRP) were first investigated for phototrophic populations using Cyanobacteria‐specific primers and 16S rRNA gene cloning. These data were expounded on by high‐throughput tagged sequencing analyses of the general bacteria population. These molecular analyses show that the microbial communities of Pavilion Lake microbialites are diverse compared to non‐lithifying microbial mats also found in the lake. Phototrophs and heterotrophs were detected, including species from the recently described Chloroacidobacteria genus, a photoheterotroph that has not been previously observed in microbialite systems. Phototrophs were shown as the most influential contributors to community differences above and below 25 meters, and corresponding shifts in heterotrophic populations were observed at this interface as well. The isotopic composition of carbonate also mirrored this shift in community states. Comparisons to previous studies indicated this population shift may be a consequence of changes in lake chemistry at this depth. Microbial community composition did not correlate with changing microbialite morphology with depth, suggesting something other than community changes may be a key to observed variations in microbialite structure. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Geobiology Wiley

Prokaryote populations of extant microbialites along a depth gradient in Pavilion Lake, British Columbia, Canada

Download PDF
15 pages

Loading next page...
 
/lp/wiley/prokaryote-populations-of-extant-microbialites-along-a-depth-gradient-cacDB49K01

References (83)

Publisher
Wiley
Copyright
Copyright © 2014 John Wiley & Sons Ltd
ISSN
1472-4677
eISSN
1472-4669
DOI
10.1111/gbi.12082
pmid
24636451
Publisher site
See Article on Publisher Site

Abstract

Pavilion Lake in British Columbia, Canada, is home to modern‐day microbialites that are actively growing at multiple depths within the lake. While microbialite morphology changes with depth and previous isotopic investigations suggested a biological role in the formation of these carbonate structures, little is known about their microbial communities. Microbialite samples acquired through the Pavilion Lake Research Project (PLRP) were first investigated for phototrophic populations using Cyanobacteria‐specific primers and 16S rRNA gene cloning. These data were expounded on by high‐throughput tagged sequencing analyses of the general bacteria population. These molecular analyses show that the microbial communities of Pavilion Lake microbialites are diverse compared to non‐lithifying microbial mats also found in the lake. Phototrophs and heterotrophs were detected, including species from the recently described Chloroacidobacteria genus, a photoheterotroph that has not been previously observed in microbialite systems. Phototrophs were shown as the most influential contributors to community differences above and below 25 meters, and corresponding shifts in heterotrophic populations were observed at this interface as well. The isotopic composition of carbonate also mirrored this shift in community states. Comparisons to previous studies indicated this population shift may be a consequence of changes in lake chemistry at this depth. Microbial community composition did not correlate with changing microbialite morphology with depth, suggesting something other than community changes may be a key to observed variations in microbialite structure.

Journal

Geobiology – Wiley

Published: Jan 1, 2014

There are no references for this article.