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Quantitative analysis of flavonoids and phenolic acid in Coreopsis tinctoria Nutt. by capillary zone electrophoresis

Quantitative analysis of flavonoids and phenolic acid in Coreopsis tinctoria Nutt. by capillary... Capillary zone electrophoresis was developed for the simultaneous determination of five flavonoids and one phenolic acid, including taxifolin‐7‐O‐glucoside, flavanomarein, quercetagetin‐7‐O‐glucoside, okanin 4′‐O‐glucoside, okanin, and chlorogenic acid, in different parts and origins of Coreopsis tinctoria and its related species. Effects of acidity, running‐buffer concentration, and modifier concentration were investigated to determine the optimum conditions for analyte determination. Analysis was performed within 18 min by using 50 mM borax buffer containing 15% acetonitrile as a modifier (pH 9.0) at 25 kV and 25°C. Hyperoside was used as internal standard for quantification. The method was accurate, simple, and repeatable, and was successfully applied to the analysis in 13 samples with satisfactory assay results. Results showed that C. tinctoria obviously differed from the related flower tea materials, “Hangju” and “Gongju”. The parts (flowers, buds, seeds, stems, and leaves) of C. tinctoria also varied among one another. This study can serve as a foundation for the quality control and pharmacological evaluation of different parts of C. tinctoria and its related species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Electrophoresis Wiley

Quantitative analysis of flavonoids and phenolic acid in Coreopsis tinctoria Nutt. by capillary zone electrophoresis

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References (24)

Publisher
Wiley
Copyright
"© 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim"
ISSN
0173-0835
eISSN
1522-2683
DOI
10.1002/elps.201700129
pmid
28681383
Publisher site
See Article on Publisher Site

Abstract

Capillary zone electrophoresis was developed for the simultaneous determination of five flavonoids and one phenolic acid, including taxifolin‐7‐O‐glucoside, flavanomarein, quercetagetin‐7‐O‐glucoside, okanin 4′‐O‐glucoside, okanin, and chlorogenic acid, in different parts and origins of Coreopsis tinctoria and its related species. Effects of acidity, running‐buffer concentration, and modifier concentration were investigated to determine the optimum conditions for analyte determination. Analysis was performed within 18 min by using 50 mM borax buffer containing 15% acetonitrile as a modifier (pH 9.0) at 25 kV and 25°C. Hyperoside was used as internal standard for quantification. The method was accurate, simple, and repeatable, and was successfully applied to the analysis in 13 samples with satisfactory assay results. Results showed that C. tinctoria obviously differed from the related flower tea materials, “Hangju” and “Gongju”. The parts (flowers, buds, seeds, stems, and leaves) of C. tinctoria also varied among one another. This study can serve as a foundation for the quality control and pharmacological evaluation of different parts of C. tinctoria and its related species.

Journal

ElectrophoresisWiley

Published: Oct 1, 2017

Keywords: ; ;

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