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Simple, sensitive and rapid liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the quantitation of Ranolazine in rat plasma

Simple, sensitive and rapid liquid chromatography/atmospheric pressure chemical ionization mass... A sensitive and specific method using liquid chromatography with atmospheric pressure chemical ionization mass spectrometry (LC/APCI‐MS) has been developed and validated for the identification and quantification of Ranolazine in rat plasma. A simple liquid–liquid extraction procedure was followed by injection of the extracts onto a C18 column with isocratic elution and detection using a single quadrupole mass spectrometer in selected ion monitoring (SIM) mode. The method was tested using six different batches of blank plasma. Linearity was established for the concentration range 0.02–10.0 µg/mL, with a coefficient of determination (r) of 0.9998 and good back‐calculated accuracy and precision. The intra‐ and inter‐day precision (relative standard deviation (RSD) %) was lower than 10%, and accuracy ranged from 85 to 115%. The lower limit of quantification was reproducible at 0.01 µg/mL with 20 µL plasma. The proposed method enables the unambiguous identification and quantification of Ranolazine for pre‐clinical and clinical studies. Copyright © 2006 John Wiley & Sons, Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Rapid Communications in Mass Spectrometry Wiley

Simple, sensitive and rapid liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the quantitation of Ranolazine in rat plasma

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References (4)

Publisher
Wiley
Copyright
Copyright © 2006 Wiley Subscription Services
ISSN
0951-4198
eISSN
1097-0231
DOI
10.1002/rcm.2331
pmid
16419025
Publisher site
See Article on Publisher Site

Abstract

A sensitive and specific method using liquid chromatography with atmospheric pressure chemical ionization mass spectrometry (LC/APCI‐MS) has been developed and validated for the identification and quantification of Ranolazine in rat plasma. A simple liquid–liquid extraction procedure was followed by injection of the extracts onto a C18 column with isocratic elution and detection using a single quadrupole mass spectrometer in selected ion monitoring (SIM) mode. The method was tested using six different batches of blank plasma. Linearity was established for the concentration range 0.02–10.0 µg/mL, with a coefficient of determination (r) of 0.9998 and good back‐calculated accuracy and precision. The intra‐ and inter‐day precision (relative standard deviation (RSD) %) was lower than 10%, and accuracy ranged from 85 to 115%. The lower limit of quantification was reproducible at 0.01 µg/mL with 20 µL plasma. The proposed method enables the unambiguous identification and quantification of Ranolazine for pre‐clinical and clinical studies. Copyright © 2006 John Wiley & Sons, Ltd.

Journal

Rapid Communications in Mass SpectrometryWiley

Published: Jan 28, 2006

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