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Surface sterilisation of phylloxera eggs for investigating grapevine‐phylloxera interactions in tissue culture

Surface sterilisation of phylloxera eggs for investigating grapevine‐phylloxera interactions in... A simple method has been developed for sterilising eggs of grape phylloxera (Daktulosphaira vitifoliae Fitch) to use in controlled experiments involving in vitro cocultivation of phylloxera with grapevine (Vitis spp.). Eggs are collected and washed with 70% ethanol followed by a sterile water rinse using a Millipore filtration apparatus. Such a filter provides a rapid means of transferring a known number of eggs into tissue culture, and subsequently determining percentage hatched. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Australian Journal of Grape and Wine Research Wiley

Surface sterilisation of phylloxera eggs for investigating grapevine‐phylloxera interactions in tissue culture

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Publisher
Wiley
Copyright
Copyright © 1999 Wiley Subscription Services, Inc., A Wiley Company
ISSN
1322-7130
eISSN
1755-0238
DOI
10.1111/j.1755-0238.1999.tb00148.x
Publisher site
See Article on Publisher Site

Abstract

A simple method has been developed for sterilising eggs of grape phylloxera (Daktulosphaira vitifoliae Fitch) to use in controlled experiments involving in vitro cocultivation of phylloxera with grapevine (Vitis spp.). Eggs are collected and washed with 70% ethanol followed by a sterile water rinse using a Millipore filtration apparatus. Such a filter provides a rapid means of transferring a known number of eggs into tissue culture, and subsequently determining percentage hatched.

Journal

Australian Journal of Grape and Wine Research – Wiley

Published: Apr 1, 1999

References

  • In vitro propagation of Dactylosphaera vitifolii ( sic ) SHIMER (Homoptera: Phylloxeridae) on shoot and root cultures of a Vitis hybrid
    Askani, Askani; Beiderbeck, Beiderbeck
  • DNA typing of populations of phylloxera ( Daktulosphaira vitifoliae (Fitch) from Australian vineyards
    Corrie, Corrie; Buchanan, Buchanan; Heeswijck, Heeswijck
  • Aseptic dual culture of grape ( Vitis spp.) and grape phylloxera ( Daktulosphaira vitifoliae FITCH)
    Forneck, Forneck; Walker, Walker; Merkt, Merkt
  • Evaluation of grape rootstocks for resistance to type A and type B grape phylloxera
    Granett, Granett; Goheen, Goheen; Lider, Lider; White, White
  • Surface sterilisation of grape phylloxera eggs in preparation for in vitro culture with Vitis species
    Grzegorczyk, Grzegorczyk; Walker, Walker
  • Evaluating resistance to grape phylloxera in Vitis species with an in vitro dual culture assay
    Grzegorczyk, Grzegorczyk; Walker, Walker
  • Zur frage der direkten oder indirekten schädigung von rebenwurzein bei befall durch die reblaus ( Daktylosphaera vitifolii ( sic ) Shimer)
    Rilling, Rilling