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Whole Mount In Situ Hybridization of Mid‐Gestation Mouse Embryos

Whole Mount In Situ Hybridization of Mid‐Gestation Mouse Embryos In situ hybridization is a powerful technique that allows the visualization of specific RNA species in biological samples in exquisite detail. It has been particularly well explored in the field of developmental genetics. The spatial and temporal patterns of RNA expression provide us with critical information on likely gene function during embryonic development, and often inform the decision on whether to attempt further gene manipulation approaches. Furthermore, once a mouse strain with altered gene function has been created, in situ hybridization is a critical tool for revealing how the development of embryos with the mutation differs from that of wild‐type embryos, and thus infer the function of the altered gene. Here, a well‐tested protocol used to visualize RNA expression in whole‐mount mid‐gestation mouse embryos ranging from 8.5 to 14.5 days post‐coitum (dpc) is described. © 2020 Wiley Periodicals LLC. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Protocols in Mouse Biology Wiley

Whole Mount In Situ Hybridization of Mid‐Gestation Mouse Embryos

Current Protocols in Mouse Biology , Volume 10 (2) – Jun 1, 2020

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References (8)

Publisher
Wiley
Copyright
© 2020 Wiley Periodicals LLC
ISSN
2161-2617
eISSN
2161-2617
DOI
10.1002/cpmo.77
Publisher site
See Article on Publisher Site

Abstract

In situ hybridization is a powerful technique that allows the visualization of specific RNA species in biological samples in exquisite detail. It has been particularly well explored in the field of developmental genetics. The spatial and temporal patterns of RNA expression provide us with critical information on likely gene function during embryonic development, and often inform the decision on whether to attempt further gene manipulation approaches. Furthermore, once a mouse strain with altered gene function has been created, in situ hybridization is a critical tool for revealing how the development of embryos with the mutation differs from that of wild‐type embryos, and thus infer the function of the altered gene. Here, a well‐tested protocol used to visualize RNA expression in whole‐mount mid‐gestation mouse embryos ranging from 8.5 to 14.5 days post‐coitum (dpc) is described. © 2020 Wiley Periodicals LLC.

Journal

Current Protocols in Mouse BiologyWiley

Published: Jun 1, 2020

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