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BRAFV600E Immunohistochemistry Facilitates Universal Screening of Colorectal Cancers for Lynch Syndrome

BRAFV600E Immunohistochemistry Facilitates Universal Screening of Colorectal Cancers for Lynch... ORIGINAL ARTICLE BRAFV600E Immunohistochemistry Facilitates Universal Screening of Colorectal Cancers for Lynch Syndrome Christopher W. Toon, FRCPA,*w Michael D. Walsh, BSc,zy Angela Chou, FRCPA,8z David Capper, MD,#** Adele Clarkson, BSc,* Loretta Sioson, BSc,* Stephen Clarke, FRACP, PhD,www Scott Mead, FRCPA, PhD,8z Rhiannon J. Walters, BAppSc,y Mark Clendenning, PhD,y Christophe Rosty, MD, PhD, FRCPA,yzzyy Joanne P. Young, PhD,y Aung Ko Win, MBBS, MPH,88 John L. Hopper, BA, MSc, PhD,88 Ashley Crook, BA, BSc, MGenCouns[MHGSA],ww Andreas von Deimling, MD,#** Mark A. Jenkins, PhD,88 Daniel D. Buchanan, PhD,y and Anthony J. Gill, MD, FRCPA*wzz MLH1 promoter methylation, and germline MLH1 mutation. Abstract: BRAFV600E mutation in microsatellite- We then assessed MMR and BRAFV600E IHC on 1403 con- unstable (MSI) colorectal carcinomas (CRCs) virtually excludes secutive CRCs. By matrix-assisted laser desorption/ionization- Lynch syndrome (LS). In microsatellite-stable (MSS) CRCs time of flight mass spectrometry 15 cases did not yield a BRAF it predicts poor prognosis. We propose a universal CRC result, whereas 38/201 (19%) were positive. By IHC 45/216 LS screening algorithm using concurrent reflex immuno- (20%) were positive. Of the 7 discordant cases, real-time PCR histochemistry (IHC) for BRAFV600E and mismatch-repair confirmed the IHC result in 6. In the 51 CRCs from the (MMR) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Surgical Pathology Wolters Kluwer Health

BRAFV600E Immunohistochemistry Facilitates Universal Screening of Colorectal Cancers for Lynch Syndrome

American Journal of Surgical Pathology , Volume 37 (10) – Oct 1, 2013

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Copyright
Copyright © 2013 by Lippincott Williams & Wilkins
ISSN
0147-5185
eISSN
1532-0979
DOI
10.1097/PAS.0b013e31828f233d
pmid
23797718
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL ARTICLE BRAFV600E Immunohistochemistry Facilitates Universal Screening of Colorectal Cancers for Lynch Syndrome Christopher W. Toon, FRCPA,*w Michael D. Walsh, BSc,zy Angela Chou, FRCPA,8z David Capper, MD,#** Adele Clarkson, BSc,* Loretta Sioson, BSc,* Stephen Clarke, FRACP, PhD,www Scott Mead, FRCPA, PhD,8z Rhiannon J. Walters, BAppSc,y Mark Clendenning, PhD,y Christophe Rosty, MD, PhD, FRCPA,yzzyy Joanne P. Young, PhD,y Aung Ko Win, MBBS, MPH,88 John L. Hopper, BA, MSc, PhD,88 Ashley Crook, BA, BSc, MGenCouns[MHGSA],ww Andreas von Deimling, MD,#** Mark A. Jenkins, PhD,88 Daniel D. Buchanan, PhD,y and Anthony J. Gill, MD, FRCPA*wzz MLH1 promoter methylation, and germline MLH1 mutation. Abstract: BRAFV600E mutation in microsatellite- We then assessed MMR and BRAFV600E IHC on 1403 con- unstable (MSI) colorectal carcinomas (CRCs) virtually excludes secutive CRCs. By matrix-assisted laser desorption/ionization- Lynch syndrome (LS). In microsatellite-stable (MSS) CRCs time of flight mass spectrometry 15 cases did not yield a BRAF it predicts poor prognosis. We propose a universal CRC result, whereas 38/201 (19%) were positive. By IHC 45/216 LS screening algorithm using concurrent reflex immuno- (20%) were positive. Of the 7 discordant cases, real-time PCR histochemistry (IHC) for BRAFV600E and mismatch-repair confirmed the IHC result in 6. In the 51 CRCs from the (MMR)

Journal

American Journal of Surgical PathologyWolters Kluwer Health

Published: Oct 1, 2013

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