Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Functional Heterogeneity of Osteopontin Isoforms in Non-small Cell Lung Cancer

Functional Heterogeneity of Osteopontin Isoforms in Non-small Cell Lung Cancer ORIGINAL ARTICLE Functional Heterogeneity of Osteopontin Isoforms in Non-small Cell Lung Cancer Chandra M. V. Goparaju, PhD, Harvey I. Pass, MD, Justin D. Blasberg, MD, Nathalie Hirsch, and Jessica S. Donington, MD Key Words: Osteopontin, Non-small cell lung cancer, mRNA Introduction: Osteopontin (OPN) is a multifunctional protein with an isoforms, Tumor progression, Epithelial-mesenchymal transition. important but poorly understood role in non-small cell lung cancer (J Thorac Oncol. 2010;5: 1516–1523) (NSCLC) pathogenesis. Moreover, the role of the three known mRNA isoforms (OPNa, OPNb, and OPNc) has not been reported. We hypothesize that OPN isoforms play different roles in determining the steopontin (OPN) is a multifunctional protein identified metastatic potential of NSCLC. Oin a remarkable range of normal and pathologic con- Methods: We amplified mRNA for each OPN isoform in NSCLC texts. It plays a key role in the mediation of immune cell tumors and matched normal lung. The functional impact of each 1,2 recruitment, wound healing, and tissue remodeling. OPN’s isoform was evaluated by transfecting cDNA plasmids specific to each diverse biologic functions involve cell adhesion, migra- isoform into NSCLC cell lines and comparing behavior to empty vector tion, and invasion, which are associated with its two highly controls in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Thoracic Oncology Wolters Kluwer Health

Functional Heterogeneity of Osteopontin Isoforms in Non-small Cell Lung Cancer

Donington, Jessica S.
Journal of Thoracic Oncology , Volume 5 (10) – Oct 1, 2010
Download PDF
8 pages

Loading next page...
 
/lp/wolters-kluwer-health/functional-heterogeneity-of-osteopontin-isoforms-in-non-small-cell-0tRfkA0Mkw

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

ISSN
1556-0864
DOI
10.1097/JTO.0b013e3181eba6bd
pmid
20689445
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL ARTICLE Functional Heterogeneity of Osteopontin Isoforms in Non-small Cell Lung Cancer Chandra M. V. Goparaju, PhD, Harvey I. Pass, MD, Justin D. Blasberg, MD, Nathalie Hirsch, and Jessica S. Donington, MD Key Words: Osteopontin, Non-small cell lung cancer, mRNA Introduction: Osteopontin (OPN) is a multifunctional protein with an isoforms, Tumor progression, Epithelial-mesenchymal transition. important but poorly understood role in non-small cell lung cancer (J Thorac Oncol. 2010;5: 1516–1523) (NSCLC) pathogenesis. Moreover, the role of the three known mRNA isoforms (OPNa, OPNb, and OPNc) has not been reported. We hypothesize that OPN isoforms play different roles in determining the steopontin (OPN) is a multifunctional protein identified metastatic potential of NSCLC. Oin a remarkable range of normal and pathologic con- Methods: We amplified mRNA for each OPN isoform in NSCLC texts. It plays a key role in the mediation of immune cell tumors and matched normal lung. The functional impact of each 1,2 recruitment, wound healing, and tissue remodeling. OPN’s isoform was evaluated by transfecting cDNA plasmids specific to each diverse biologic functions involve cell adhesion, migra- isoform into NSCLC cell lines and comparing behavior to empty vector tion, and invasion, which are associated with its two highly controls in

Journal

Journal of Thoracic Oncology – Wolters Kluwer Health

Published: Oct 1, 2010

References

  • Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity
    Ashkar
  • Altered wound healing in mice lacking a functional osteopontin gene (spp1)
    Liaw
  • Osteopontin: it's role in regulation of cell motility and nuclear factor kappa B-mediated urokinase type plasminogen activator expression
    Das
  • Osteopontin: a protein with diverse functions
    Denhardt
  • Reduced malignancy of ras-transformed NIH 3T3 cells expressing antisense osteopontin RNA
    Behrend
  • Specific reduction in osteopontin synthesis by antisense RNA inhibits the tumorigenicity of transformed Rat1 fibroblasts
    Gardner
  • Osteopontin: a key cytokine in cell-mediated and granulomatous inflammation
    O'Regan
  • cDNA cloning, mRNA distribution and heterogeneity, chromosomal location, and RFLP analysis of human osteopontin (OPN)
    Young
  • Overexpression of osteopontin is associated with more aggressive phenotypes in human non-small cell lung cancer
    Hu
  • Vascular endothelial growth factor and osteopontin in stage I lung adenocarcinoma
    Shijubo
  • Osteopontin expression and prognostic significance in non-small cell lung cancer
    Donati
  • Lung cancer osteopontin isoforms exhibit angiogenic functional heterogeneity
    Blasberg
  • Summaries of Affymetrix GeneChip probe level data
    Irizarry
  • The epithelial-mesenchymal transition: new insights in signaling, development, and disease
    Lee
  • Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method
    Livak
  • An evaluation of tumor oxygenation and gene expression in patients with early stage non-small cell lung cancers
    Le
  • Differential osteopontin expression in lung cancer
    Zhang
  • Axis of evil: molecular mechanisms of cancer metastasis
    Bogenrieder
  • Expression of osteopontin in human glioma. Its correlation with the malignancy
    Saitoh
  • Osteopontin-c is a selective marker of breast cancer
    Mirza
  • An osteopontin splice variant induces anchorage independence in human breast cancer cells
    He
  • Distinct mechanisms of tumor invasion and metastasis
    Yilmaz
  • N-cadherin signaling potentiates mammary tumor metastasis via enhanced extracellular signal-regulated kinase activation
    Hulit
  • Differential cadherin expression: potential markers for epithelial to mesenchymal transformation during tumor progression
    Agiostratidou