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Immunohistochemistry Is Highly Sensitive and Specific for the Detection of V600E BRAF

Immunohistochemistry Is Highly Sensitive and Specific for the Detection of V600E BRAF ORIGINAL ARTICLE Immunohistochemistry Is Highly Sensitive and Specific for the Detection of V600E BRAF Mutation in Melanoma Georgina V. Long, MD, PhD, FRACP,*wz James S. Wilmott, BSc,*y David Capper, MD,8z Matthias Preusser, MD,8# Yuxiao E. Zhang, MBiotech,* John F. Thompson, MD, FRACS, FACS,***ww Richard F. Kefford, MBBS, PhD, FRACP,*wz Andreas von Deimling, MD,8z and Richard A. Scolyer, MD, FRCPA, FRCPath*yzz status by independent, blinded observers using both conven- Abstract: This study investigated the sensitivity and specificity of tional DNA molecular techniques and IHC with the novel immunohistochemical (IHC) analysis using an anti-BRAF an- BRAF V600E mutant-specific antibody, VE1. The antibody had tibody to detect the presence of the BRAF V600E mutation in a sensitivity of 97% (37/38) and a specificity of 98% (58/59) for patients with metastatic melanoma. A total of 100 patients with detecting the presence of a BRAF V600E mutation. Of the American Joint Committee on Cancer stage IIIC unresectable or BRAF-mutated cases, none of the non-V600E cases (including stage IV melanoma and who underwent tumor DNA BRAF V600K) stained positive with the antibody (0/11). There were 5 mutation testing were selected. Paraffin-embedded, formalin- cases with discordant BRAF mutation results. Additional mo- fixed melanoma biopsies were analyzed http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Surgical Pathology Wolters Kluwer Health

Immunohistochemistry Is Highly Sensitive and Specific for the Detection of V600E BRAF

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References (27)

Copyright
Copyright © 2012 by Lippincott Williams & Wilkins
ISSN
0147-5185
eISSN
1532-0979
DOI
10.1097/PAS.0b013e31826485c0
pmid
23026937
Publisher site
See Article on Publisher Site

Abstract

ORIGINAL ARTICLE Immunohistochemistry Is Highly Sensitive and Specific for the Detection of V600E BRAF Mutation in Melanoma Georgina V. Long, MD, PhD, FRACP,*wz James S. Wilmott, BSc,*y David Capper, MD,8z Matthias Preusser, MD,8# Yuxiao E. Zhang, MBiotech,* John F. Thompson, MD, FRACS, FACS,***ww Richard F. Kefford, MBBS, PhD, FRACP,*wz Andreas von Deimling, MD,8z and Richard A. Scolyer, MD, FRCPA, FRCPath*yzz status by independent, blinded observers using both conven- Abstract: This study investigated the sensitivity and specificity of tional DNA molecular techniques and IHC with the novel immunohistochemical (IHC) analysis using an anti-BRAF an- BRAF V600E mutant-specific antibody, VE1. The antibody had tibody to detect the presence of the BRAF V600E mutation in a sensitivity of 97% (37/38) and a specificity of 98% (58/59) for patients with metastatic melanoma. A total of 100 patients with detecting the presence of a BRAF V600E mutation. Of the American Joint Committee on Cancer stage IIIC unresectable or BRAF-mutated cases, none of the non-V600E cases (including stage IV melanoma and who underwent tumor DNA BRAF V600K) stained positive with the antibody (0/11). There were 5 mutation testing were selected. Paraffin-embedded, formalin- cases with discordant BRAF mutation results. Additional mo- fixed melanoma biopsies were analyzed

Journal

American Journal of Surgical PathologyWolters Kluwer Health

Published: Jan 1, 2013

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