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Mechanisms by Which Adenosine Restores Conduction in Dormant Canine Pulmonary Veins

Mechanisms by Which Adenosine Restores Conduction in Dormant Canine Pulmonary Veins Arrhythmia/Electrophysiology Mechanisms by Which Adenosine Restores Conduction in Dormant Canine Pulmonary Veins Toma´s Datino, MD, PhD; Laurent Macle, MD; Xiao-Yan Qi, PhD; Ange Maguy, PhD; Philippe Comtois, PhD; Denis Chartier, BSc; Peter G. Guerra, MD; Angel Arenal, MD; Francisco Ferna´ndez-Avile´s, MD, PhD; Stanley Nattel, MD Background—Adenosine acutely reconnects pulmonary veins (PVs) after radiofrequency application, revealing “dormant conduction” and identifying PVs at risk of reconnection, but the underlying mechanisms are unknown. Methods and Results—Canine PV and left-atrial (LA) action potentials were recorded with standard microelectrodes and ionic currents with whole-cell patch clamp before and after adenosine perfusion. PVs were isolated with radiofrequency current application in coronary-perfused LA-PV preparations. Adenosine abbreviated action potential duration similarly in PV and LA but significantly hyperpolarized resting potential (by 3.90.5%; P0.05) and increased dV/dt (by max 3410%) only in PV. Increased dV/dt was not due to direct effects on I , which was reduced similarly by adenosine max Na in LA and PV but correlated with resting-potential hyperpolarization (r0.80). Adenosine induced larger inward rectifier K current (I ) in PV (eg, –2.280.04 pA/pF; –100 mV) versus LA (–1.280.16 pA/pF). Radiofrequency KAdo ablation isolated PVs by depolarizing resting potential to voltages positive to –60 mV. Adenosine http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Circulation Wolters Kluwer Health

Mechanisms by Which Adenosine Restores Conduction in Dormant Canine Pulmonary Veins

Circulation , Volume 121 (8) – Mar 1, 2010

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References (33)

ISSN
0009-7322
eISSN
1524-4539
DOI
10.1161/CIRCULATIONAHA.109.893107
pmid
20159830
Publisher site
See Article on Publisher Site

Abstract

Arrhythmia/Electrophysiology Mechanisms by Which Adenosine Restores Conduction in Dormant Canine Pulmonary Veins Toma´s Datino, MD, PhD; Laurent Macle, MD; Xiao-Yan Qi, PhD; Ange Maguy, PhD; Philippe Comtois, PhD; Denis Chartier, BSc; Peter G. Guerra, MD; Angel Arenal, MD; Francisco Ferna´ndez-Avile´s, MD, PhD; Stanley Nattel, MD Background—Adenosine acutely reconnects pulmonary veins (PVs) after radiofrequency application, revealing “dormant conduction” and identifying PVs at risk of reconnection, but the underlying mechanisms are unknown. Methods and Results—Canine PV and left-atrial (LA) action potentials were recorded with standard microelectrodes and ionic currents with whole-cell patch clamp before and after adenosine perfusion. PVs were isolated with radiofrequency current application in coronary-perfused LA-PV preparations. Adenosine abbreviated action potential duration similarly in PV and LA but significantly hyperpolarized resting potential (by 3.90.5%; P0.05) and increased dV/dt (by max 3410%) only in PV. Increased dV/dt was not due to direct effects on I , which was reduced similarly by adenosine max Na in LA and PV but correlated with resting-potential hyperpolarization (r0.80). Adenosine induced larger inward rectifier K current (I ) in PV (eg, –2.280.04 pA/pF; –100 mV) versus LA (–1.280.16 pA/pF). Radiofrequency KAdo ablation isolated PVs by depolarizing resting potential to voltages positive to –60 mV. Adenosine

Journal

CirculationWolters Kluwer Health

Published: Mar 1, 2010

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